Resolution and Enhancement in Nanoantenna-Based Fluorescence Microscopy
Resolution and Enhancement in Nanoantenna-Based Fluorescence Microscopy
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DOI：
10.1021/nl902183y
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发表时间：
2009-12-01
期刊：
影响因子：
10.8
通讯作者：
Sandoghdar, Vahid
中科院分区：
文献类型：
--
作者：
Eghlidi, Hadi;Lee, Kwang Geol;Sandoghdar, Vahid
单个金纳米颗粒可以作为纳米天线，用于增强发射体在其近场中的荧光。在这里，我们提出了实验和理论研究的扫描天线为基础的荧光显微镜作为金纳米粒子的直径的函数。我们研究荧光增强和空间分辨率之间的相互作用，并讨论了在致密样品中破译单分子的要求。实验证明，分辨率优于20 nm和荧光增强高达30倍。通过在时域有限差分计算中考虑尖端轴和样品界面，我们解释了为什么测得的荧光增强在界面的存在下比均匀环境中预测的值更高。
Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the diameter of the gold nanoparticle. We examine the interplay between fluorescence enhancement and spatial resolution and discuss the requirements for deciphering single molecules in a dense sample. Resolutions better than 20 nm and fluorescence enhancement up to 30 times are demonstrated experimentally. By accounting for the tip shaft and the sample interface in finite-difference time-domain calculations, we explain why the measured fluorescence enhancements are higher in the presence of an interface than the values predicted for a homogeneous environment.