Multiplex protein assays based on real-time magnetic nanotag sensing
Multiplex protein assays based on real-time magnetic nanotag sensing
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DOI：
10.1073/pnas.0810822105
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发表时间：
2008-12-30
影响因子：
11.1
通讯作者：
Wang, Shan X.
中科院分区：
文献类型：
--
作者：
Osterfeld, Sebastian J.;Yu, Heng;Wang, Shan X.
磁性纳米标签 (MNT) 是生物分子检测分析中荧光标记的一种有前途的替代品，因为可以使用廉价的巨磁阻 (GMR) 传感器（例如自旋阀 (SV) 传感器）检测微量的 MNT。然而，将这一前景转化为易于使用和多重蛋白质检测（在癌症诊断和治疗监测等分子诊断中备受追捧）一直具有挑战性。在这里，我们展示了在亚皮摩尔浓度水平和超过四十年的动态范围内对潜在癌症标志物的多重蛋白质检测。通过添加纳米标签扩增，分析灵敏度扩展到低 fM 浓度范围。基于 MNT 的蛋白质检测技术的多分析能力、灵敏度、可扩展性和易用性使其成为研究和临床环境中多功能和便携式分子诊断的有力竞争者。
Magnetic nanotags (MNTs) are a promising alternative to fluorescent labels in biomolecular detection assays, because minute quantities of MNTs can be detected with inexpensive giant magnetoresistive (GMR) sensors, such as spin valve (SV) sensors. However, translating this promise into easy to use and multilplexed protein assays, which are highly sought after in molecular diagnostics such as cancer diagnosis and treatment monitoring, has been challenging. Here, we demonstrate multiplex protein detection of potential cancer markers at subpicomolar concentration levels and with a dynamic range of more than four decades. With the addition of nanotag amplification, the analytic sensitivity extends into the low fM concentration range. The multianalyte ability, sensitivity, scalability, and ease of use of the MNT-based protein assay technology make it a strong contender for versatile and portable molecular diagnostics in both research and clinical settings.