A chitinase-like protein in the lung and circulation of patients with severe asthma
A chitinase-like protein in the lung and circulation of patients with severe asthma
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DOI：
10.1056/nejmoa073600
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发表时间：
2007-11-15
影响因子：
158.5
通讯作者：
Elias, Jack A.
中科院分区：
文献类型：
--
作者：
Chupp, Geoffrey L.;Lee, Chun Geun;Elias, Jack A.
背景资料：进化上保守的18-糖基水解酶家族包含真正的几丁质酶和缺乏酶活性的几丁质酶样蛋白。酸性哺乳动物几丁质酶最近与哮喘动物模型有关。相关的几丁质酶样蛋白YKL-40（也称为人软骨糖蛋白39 [HCgp-39]和几丁质酶3样1）可以在血清中容易地测量。然而，它与哮喘的关系尚未evaluated.Methods：我们定量血清YKL-40水平在三个队列的哮喘患者招募的患者人群在耶鲁大学，一个从巴黎大学，一个从威斯康星大学，以及从周围社区的控制。在巴黎队列中，使用免疫组织化学分析和形态定量来评估YKL-40在肺部的表达位点。结果：哮喘患者血清YKL-40水平显著高于对照组，肺组织YKL-40水平显著高于对照组，肺组织YKL-40水平显著高于对照组，肺组织YKL-40水平显著高于对照组。在巴黎队列中，肺YKL-40水平升高，并与循环YKL-40水平相关（r=0.55，P
Background: The evolutionarily conserved 18-glycosyl-hydrolase family contains true chitinases and chitinase-like proteins that lack enzymatic activity. Acidic mammalian chitinase has recently been associated with animal models of asthma. The related chitinase-like protein, YKL-40 (also called human cartilage glycoprotein 39 [HCgp-39] and chitinase 3-like 1), can be readily measured in the serum. However, its relationship to asthma has not been evaluated.Methods: We quantified serum YKL-40 levels in three cohorts of patients with asthma-one recruited from the patient population at Yale University, one from the University of Paris, and one from the University of Wisconsin-as well as in controls from the surrounding communities. In the Paris cohort, immunohistochemical analysis and morphometric quantitation were used to evaluate the locus of expression of YKL-40 in the lung. The clinical characteristics of the patients with high serum or lung YKL-40 levels were also evaluated.Results: Serum YKL-40 levels were significantly elevated in patients with asthma as compared with controls. In the Paris cohort, lung YKL-40 levels were elevated and were correlated with circulating YKL-40 levels (r=0.55, P