MEIOTIC COMPETENCE ACQUISITION IS ASSOCIATED WITH THE APPEARANCE OF M-PHASE CHARACTERISTICS IN GROWING MOUSE OOCYTES
MEIOTIC COMPETENCE ACQUISITION IS ASSOCIATED WITH THE APPEARANCE OF M-PHASE CHARACTERISTICS IN GROWING MOUSE OOCYTES
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DOI：
10.1016/0012-1606(91)90063-9
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发表时间：
1991-01-01
影响因子：
2.7
通讯作者：
ALBERTINI, DF
中科院分区：
文献类型：
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作者：
WICKRAMASINGHE, D;EBERT, KM;ALBERTINI, DF
为了确定是否收购减数分裂能力在卵子发生的生长阶段与M期特征的外观，从13日龄至30日龄的小鼠获得的卵母细胞进行了评价，通过荧光显微镜染色质和微管组织，在体外成熟能力，和M期磷蛋白的分布。减数分裂不称职的卵母细胞从他们的主管同行在显示精心设计的间期样阵列的细胞质微管和分散germinal囊泡染色质的区别。减数分裂有能力的卵母细胞的大小较大，表现出浓缩的染色质周围的核仁，并显示出与多个微管组织中心的外观相结合的细胞质微管进行性减少。培养24小时后，中到大尺寸的卵母细胞表现出核仁周围染色质凝聚恢复减数分裂，而较小的减数分裂不胜任的卵母细胞保留GV与弥漫的染色质。此外，间接免疫荧光研究使用M期磷蛋白特异性单克隆抗体MPM-2表明，反应性胞质病灶的外观直接与减数分裂能力的卵母细胞的核变化特征。因此，在未成熟小鼠卵巢中卵母细胞生长期间最早过渡到减数分裂能力状态的特征在于核和细胞质组分的阶段特异性和协调修饰。
To determine whether the acquisition of meiotic competence during the growth phase of oogenesis is associated with the appearance of M-phase characteristics, oocytes obtained from 13- to 30-day-old mice were evaluated by fluorescence microscopy with respect to chromatin and microtubule organization,in vitromaturation ability, and the distribution of M-phase phosphoproteins. Meiotically incompetent oocytes were distinguished from their competent counterparts in displaying elaborate interphase-like arrays of cytoplasmic microtubules and dispersed germinal vesicle chromatin. Meiotically competent oocytes were larger in size, exhibited condensation of chromatin around the nucleolus, and displayed a progressive diminution of cytoplasmic microtubules in conjunction with the appearance of multiple microtubule organizing centers. After 24 hr in culture, medium- to large-sized oocytes exhibiting perinucleolar chromatin condensation resume meiosis whereas smaller meiotically incompetent oocytes retain GVs with diffuse chromatin. Moreover, indirect immunofluorescence studies using the M-phase phosphoprotein specific monoclonal antibody MPM-2 indicate that the appearance of reactive cytoplasmic foci is directly correlated with nuclear changes characteristic of meiotically competent oocytes. Thus, the earliest transition to a meiotically competent state during oocyte growth in the immature mouse ovary is characterized by stage-specific and coordinated modifications of nuclear and cytoplasmic components.