Cell interactions in the sea urchin embryo studied by fluorescence photoablation.
Cell interactions in the sea urchin embryo studied by fluorescence photoablation.
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通过荧光光消融研究海胆胚胎中的细胞相互作用。
DOI：
10.1126/science.2188366
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Ettensohn,CA
中科院分区：
文献类型：
--
作者：
Ettensohn,CA
在许多生物体中，细胞之间的相互作用在细胞命运的规范中起着关键作用。在海胆胚胎中，初级间充质细胞（PMC）调节次级间充质细胞（SMC）亚群的发育程序。这种细胞相互作用的时间进行了分析，通过荧光光消融技术，这是用来专门消融PMC在不同的发展阶段。此外，将PMC显微注射到PMC耗尽的受体胚胎在不同的发育阶段，并检查其对SMC命运的影响。PMC和SMC之间的关键相互作用是短暂的，发生在原肠胚形成后期。在此之前，SMC对PMC传递的抑制信号不敏感。
In many organisms, interactions between cells play a critical role in the specification of cell fates. In the sea urchin embryo, primary mesenchyme cells (PMCs) regulate the developmental program of a subpopulation of secondary mesenchyme cells (SMCs). The timing of this cell interaction was analyzed by means of a fluorescence photoablation technique, which was used to specifically ablate PMCs at various stages of development. In addition, the PMCs were microinjected into PMC-depleted recipient embryos at different developmental stages and their effect on SMC fate was examined. The critical interaction between PMCs and SMCs was brief and took place late in gastrulation. Before that time, SMCs were insensitive to the suppressive signals transmitted by the PMCs.