Collection of genomic DNA by the noninvasive mouthwash method for use in pharmacogenetic studies
Collection of genomic DNA by the noninvasive mouthwash method for use in pharmacogenetic studies
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DOI：
10.1592/phco.22.12.954.33598
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发表时间：
2002-08-01
期刊：
影响因子：
4.1
通讯作者：
Johnson, JA
中科院分区：
文献类型：
--
作者：
Andrisin, TE;Humma, LM;Johnson, JA
研究目的。为了确定通过漱口水方法在口腔细胞中收集的基因组DNA样品的长期稳定性、数量和质量，用于药物遗传学研究，设计。前瞻性分析。设置。佛罗里达的大学药物基因组学中心和波多黎各及美国的医学中心参与了一项多中心国际试验。药物基因组学中心的10名志愿者和正在进行的多中心临床试验的2011名参与者。基因组DNA的稳定性通过测量来自漱口水样品的DNA产率来确定，所述漱口水样品从六名志愿者获得并在室温下储存超过90天，所述漱口水样品从201个临床试验样品获得，所述临床试验样品在室温下储存和运输。在四名志愿者中评估了三次5 ml漱口水冲洗与一次10 ml漱口水冲洗的DNA产量是否更高。在稳定性研究中，通过聚合酶链反应（PCR）检测和基因分型确定DNA扩增的成功率，对从6名志愿者中随机选择的32份样本的基因组DNA质量进行了评估。对于稳定性研究，基因组DNA的量随着在室温下储存的时间而减少（总体p <0.05）。
Study Objective. To determine long-term stability, quantity, and quality of genomic DNA samples collected in buccal cells by the mouthwash method, for use in pharmacogenetic studies,Design. Prospective analysis.Setting. University pharmacogenomics center in Florida and medical centers in Puerto Rico and the United States participating in a multicenter international trial.Subjects. Ten volunteers at the pharmacogenomics center and 2011 participants in the ongoing multicenter clinical trial.Intervention. Stability of genomic DNA was determined by measuring DNA yield from mouthwash samples obtained from Six Volunteers and stored at room temperature over 90 days and from 201 clinical trial samples that were stored and shipped at room temperature. Whether DNA yield was higher with three 5-ml mouthwash rinses versus one 10-ml rinse was evaluated in four volunteers. Quality, of genomic DNA was assessed on 32 randomly selected samples from the six volunteers in the stability study by determining the success rate of DNA amplification with polymerase chain reaction (PCR) testing and by genotyping.Measurements and Main Results. For the stability studies, the quantity of genomic DNA decreased over time with storage at room temperature (overall p