Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms
Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms
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DOI：
10.1089/mdr.1995.1.255
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发表时间：
1995-09-01
期刊：
影响因子：
--
通讯作者：
Aarestrup, FM
中科院分区：
文献类型：
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作者：
Aarestrup, FM
肠球菌已成为医院获得性菌血症的重要原因。 11, 13 在过去 20 年中，观察到肠球菌的抗菌药物耐药性有所增加，7-1' 并且随着万古霉素耐药性的出现，肠球菌现在可能对所有目前批准的抗菌药物都具有耐药性。 4-8-11 最近的研究报告了环境和家养动物中存在耐万古霉素肠球菌。 1, 9, 14 此外，有人提出对万古霉素（一种用于人类治疗的糖肽）的耐药性与用作动物饲料添加剂的糖肽阿伏霉素之间存在关联（图 I）。 9 本研究报告了丹麦传统家禽养殖场和生态家禽养殖场分离出的屎肠球菌对糖肽耐药性的检测。从 8 个传统家禽养殖场的肉鸡群和 6 个生态养殖场的蛋禽中采集了 5 个新鲜排泄的粪便样本。所有传统农场使用的家禽饲料均含有阿伏霉素作为饲料添加剂，浓度为 15 毫克/千克。生态农场不使用抗生素作为饲料添加剂或用于治疗。用无菌棉签将粪便直接划线到牛血琼脂平板上（哥伦比亚琼脂，CM 331，含有5%无菌牛血），补充七叶灵并在37°C需氧培养过夜。通过目视检查细菌菌落的形态来评估原代培养物。根据 Devriese 等人的说法，从每个平板中对一个疑似肠球菌的细菌菌落进行传代培养和鉴定。 5 总共13英镑。粪肠菌从 8 个传统农场分离出来，售价 16 英镑。鉴定出来自 6 个生态农场的粪便分离株。通过片剂扩散试验测试了所有 29 株分离株对糖肽的敏感性。
ENTEROCOCCihave emerged as important causes of hospi-tal-acquired bacteremia. 11, 13 During the past 20 years an increase in antimicrobial resistance among enterococci has been observed, 7-1'and with the emergence of vancomycin resistance, enterococci may now be resistant to all currently approved an-timicrobial agents. 4-8-11 Recent studies have reported van-comycin-resistant enterococci in the environment and in domestic production animals. 1, 9, 14 Furthermore, an association between resistance to vancomycin, a glycopeptide used thera-peutically in humans, and the glycopeptide avoparcin, which is used as a feed additive for animals, has been suggested (Fig. I). 9 The present study reports the detection of glycopeptide re-sistance among E. faecium isolates from conventional and eco-logical poultry farms in Denmark. Five freshly voided fecal samples were collected from broiler flocks on each of 8 conventional poultry farms and from laying birds on each of 6 ecological farms. The poultry feed used on all the conventional farms included avoparcin as a feed additive in concentrations of 15 mg/kg. No antibiotics were used either as a feed additive or therapeutically on the ecological farms. With a sterile cotton swab feces was streaked directly onto bovine blood agar plates (Columbia agar, CM 331, with 5% sterile bovine blood), supplemented with aesculin and incubated aerobically at 37 C overnight. Primary cultures were evaluated by visual examination of the morphology of the bacterial colonies. From each plate one bacterial colony suspected to be enterococci was subcultured and identified according to Devriese et al. 5 A total of 13£. faecium isolates from the 8 conventional farms and 16£. faecium isolates from the 6 eco-logical farms were identified. All 29 isolates were tested for susceptibility to glycopeptides by a tablet diffusion test on