Boron Doped Diamond Electrodes for Direct Measurement in Biological Fluids: An In Situ Regeneration Approach
Boron Doped Diamond Electrodes for Direct Measurement in Biological Fluids: An In Situ Regeneration Approach
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DOI：
10.1149/2.014302jes
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发表时间：
2013-01-01
影响因子：
3.9
通讯作者：
Bergonzo, Philippe
中科院分区：
文献类型：
--
作者：
Kiran, Raphael;Scorsone, Emmanuel;Bergonzo, Philippe
硼掺杂金刚石（BDD）电极由于其具有独特的组合性能，在生物医学领域的应用前景非常广阔。尽管有这些优点，BDD电极在生物液体（尿液、血浆）和合成液体中使用时容易结垢。我们提出了一种电化学（EC）处理方法，其中一列短阴极和/或阳极脉冲应用于清洁的污染电极。该技术可用于恢复硼掺杂金刚石电极失去的反应性，以电子转移率k(0)为特征，从而提高其在长时间测量中的可重用性，而不会降低信号，从而显着扩展了监测和测量应用领域。该技术不需要使用特定的介质，因此可以直接在探测流体中进行。虽然含有非电活性物质的水电解质是EC活化的首选，但它也可以在生物流体中进行，如血液、尿液等，从而开辟了体内分析的领域。通过电化学阻抗谱（EIS）观察到活化过程后k(0)值提高到0.1 cm s（-1）。该技术在很大程度上提高了电极的灵敏度、再现性和寿命。(C) 2012电化学学会。[DOI: 10.1149/2.014302jes]版权所有
Boron doped diamond (BDD) electrodes are extremely promising in the field of biomedical applications as they exhibit a unique combination of properties. Despite these advantages, BDD electrodes are prone to fouling when used in biological fluids (urine, blood plasma), and synthetic fluids. We propose a electrochemical (EC) treatment where a train of short cathodic and/or anodic pulses are applied to clean fouled electrodes. This technique can be used to retrieve the lost reactivity, characterized by electron transfer rate k(0) of the boron doped diamond electrodes, thereby enhancing their reusability over long period of measurements without degradation of the signal, thus significantly extending the field of monitoring and surveying applications. The technique does not require the use of a specific medium and thus can be directly performed in the probed fluid. Although an aqueous electrolyte containing non-electroactive species is preferred for EC activation, it can also be done in biological fluids such as blood, urine etc, thereby opening the field for in-vivo analysis. Through Electrochemical impedance spectroscopy (EIS) it was observed that the k(0) value was increased up to 0.1 cm s(-1) after the activation process. This technique improves the sensitivity, reproducibility and lifetime of the electrodes to a considerable extent. (C) 2012 The Electrochemical Society. [DOI: 10.1149/2.014302jes] All rights reserved.