Differentiation of preosteoblasts using a delivery system with BMPs and bioactive glass microspheres
Differentiation of preosteoblasts using a delivery system with BMPs and bioactive glass microspheres
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DOI：
10.1007/s10856-006-0687-4
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发表时间：
2007-02-01
影响因子：
3.7
通讯作者：
Faucheux, N.
中科院分区：
文献类型：
--
作者：
Bergeron, E.;Marquis, M. E.;Faucheux, N.
骨形态发生蛋白（BMP）和45 S5生物玻璃（R）微球（生物活性GM）可以增加成骨细胞的分化。重组人骨形态发生蛋白-2（rhBMP-2）是目前最常用的骨形态发生蛋白载体，并已用于临床骨愈合研究。我们已经开发出一种结合I型胶原凝胶、BMP和生物活性GM的递送系统。由于BMP-9似乎比BMP-2更成骨，我们比较了由我们的递送系统诱导的MC 3 T3-E1前成骨细胞的分化，所述递送系统含有源自BMP-9的肽（pBMP-9）或rhBMP-2，两者均为100 ng/mL。5天后，碱性磷酸酶染色显示，在所有实验条件下，pBMP-9比rhBMP-2诱导更多的分化。此外，生物活性GM增加了这种BMP效应。由于前成骨细胞分泌的基质金属蛋白酶（MMPs），可以降解胶原蛋白，然后我们研究了输送系统对MMPs生产的影响。我们观察到MMP-2是所有实验条件下参与的主要MMP。此外，在第3天和第5天，具有生物活性GM的pBMP-9产生的MMP-2比rhBMP-2少。因此，使用I型胶原凝胶与pBMP-9和生物活性GM的递送系统似乎是用于骨再生的有前景的系统。
Bone morphogenetic proteins (BMPs) and 45S5 Bioglass (R) microspheres (bioactive GM) can increase the differentiation of osteoblasts. Recombinant human BMP-2 (rhBMP-2) is presently the BMP most frequently used in delivery systems and it has already been used in clinical bone healing studies. We have developed a delivery system that combines a collagen Type I gel, BMP and bioactive GM. Since BMP-9 seems to be more osteogenic than BMP-2, we compared the differentiation of MC3T3-E1 preosteoblasts induced by our delivery system containing either a peptide derived from BMP-9 (pBMP-9), or rhBMP-2, both at 100 ng/mL. After 5 days, alkaline phosphatase staining showed that pBMP-9 induced more differentiation than rhBMP-2 in all experimental conditions. Also, bioactive GM increased this BMP effect. Since preosteoblasts secreted matrix metalloproteinases (MMPs) that can degrade collagen, we then studied the influence of the delivery system on MMPs production. We observed that MMP-2 was the major MMP involved in all experimental conditions. In addition, pBMP-9 with bioactive GM generated less MMP-2 than did rhBMP-2 on days 3 and 5. Thus, a delivery system using collagen Type I gel with pBMP-9 and bioactive GM seems to be a promising system for bone regeneration.