Chromosomal assignment of the human homologues of feline sarcoma virus and avian myeloblastosis virus onc genes.
Chromosomal assignment of the human homologues of feline sarcoma virus and avian myeloblastosis virus onc genes.
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猫肉瘤病毒和禽成髓细胞瘤病毒 onc 基因的人类同源物的染色体分配。
DOI：
10.1073/pnas.79.15.4714
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发表时间：
1982
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Dalla-Favera,R;Franchini,G;Martinotti,S;Wong-Staal,F;Gallo,RC;Croce,CM
逆转录病毒转化基因，v-onc基因，来源于正常细胞序列，称为细胞onc（c-onc）基因。来自选择性丢失人类染色体的小鼠-人类体细胞杂交体的DNA用于Southern印迹以绘制两个人类onc基因的染色体位置。使用逆转录病毒onc基因的克隆人类同源物作为探针。因为人类c-fes基因，这是同源的猫肉瘤病毒，与人类15号染色体一致，和人类c-myb基因，这是同源的禽成髓细胞瘤病毒onc基因，与人类6号染色体一致，我们已经分配的c-fes和c-myb基因，分别到人类15号和6号染色体。在肿瘤中观察到涉及这些人类染色体的非随机染色体缺陷。这些研究应该是有价值的，以确定是否涉及这些染色体的特定重排导致这些onc基因在人类恶性肿瘤的异常表达。
Retroviral transforming genes, v-onc genes, are derived from normal cellular sequences that are called cellular onc (c-onc) genes. DNA from mouse-human somatic cell hybrids that have selectively lost human chromosomes was used in Southern blots to map the chromosomal location of two human onc genes. Cloned human homologues of retroviral onc genes were used as probes. Because the human c-fes gene, which is homologous to feline sarcoma virus, segregates concordantly with human chromosome 15, and the human c-myb gene, which is homologous to avian myeloblastosis virus onc genes, segregates concordantly with human chromosome 6, we have assigned the c-fes and the c-myb genes to human chromosomes 15 and 6, respectively. Nonrandom chromosomal defects involving these human chromosomes have been observed in neoplasms. These studies should be valuable in determining whether specific rearrangements involving these chromosomes result in the abnormal expression of these onc genes in human malignancies.