Genome-Wide Analysis of NAC Gene Family in Betula pendula
Genome-Wide Analysis of NAC Gene Family in Betula pendula
复制标题
垂枝桦 NAC 基因家族的全基因组分析
DOI：
10.3390/f10090741
复制
发表时间：
2019-09-01
期刊：
影响因子：
2.9
通讯作者：
Chen, Su
中科院分区：
文献类型：
--
作者：
Chen, Song;Lin, Xin;Chen, Su
NACS（NAM，ATAF1/2和CUC2）是植物特异性转录因子，在各种植物发育过程中起着各种作用。在这项研究中，我们鉴定了桦木中的NAC基因家族（Betula Pendula），并进一步分析了BPNAC的功能。系统发育分析表明，114个BPNAC可以分为七个亚家族。我们研究了这些BPNAC在桦木不同组织中的表达水平，包括根，木质部，叶子和花朵，结果表明，在木质部和根中，BPNAC似乎比叶子和花朵更高。除了组织特异性表达分析外，我们还研究了低温应激下BPNAC的表达。在低温应力下，总共21个BPNAC被差异表达，其中17个被上调，四个被下调。使用基因表达数据，我们重建了21个低温反应性BPNAC的基因共表达网络。总之，我们的结果提供了对B. pendula基因组中NAC基因演变的见解，并为理解BPNAC介导的桦木中的冷反应的分子机制提供了基础。
NACs (NAM, ATAF1/2, and CUC2) are plant-specific transcription factors that play diverse roles in various plant developmental processes. In this study, we identified the NAC gene family in birch (Betula pendula) and further analyzed the function of BpNACs. Phylogenetic analysis reveals that the 114 BpNACs can be divided into seven subfamilies. We investigated the expression levels of these BpNACs in different tissues of birch including roots, xylem, leaves, and flowers, and the results showed that the BpNACs seem to be expressed higher in xylem and roots than leaves and flowers. In addition to tissue-specific expression analysis, we investigated the expression of BpNACs under low-temperature stress. A total of 21 BpNACs were differentially expressed under low-temperature stress, of which 17 were up-regulated, and four were down-regulated. Using the gene expression data, we reconstructed the gene co-expression network for the 21 low-temperature-responsive BpNACs. In conclusion, our results provide insight into the evolution of NAC genes in the B. pendula genome, and provide a basis for understanding the molecular mechanism for BpNAC-mediated cold responses in birch.