Efficient Gene Delivery Based on Guanidyl-Nucleic Acid Molecular Interactions
Efficient Gene Delivery Based on Guanidyl-Nucleic Acid Molecular Interactions
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DOI：
10.1002/adfm.202004783
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Min Liu
中科院分区：
文献类型：
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作者：
Dongli Wang;Jie Song;Jing Wang;Zhiyi Zhang;Qing Shen;Jun Wang;Haiyan Guo;Ruifeng Wang;Cao Xie;Weiyue Lu;Min Liu
Low transfection efficacy of non-viral gene vectors restricts their applications..In this paper, N1,N3-dicarbamimidoyl-5-methylisophthalamide (BGG).is designed as a functional group, in which two guanidyls are located at the.meta positions of an aryl ring. BGG is conjugated with PAMAM G5.(G5-BGG) and G5-BGG/DNA complex is dispersed into a polyvinyl alcohol.(PVA) hydrogel for local injection. Molecular docking, NMR, IR and Isothermal.titration calorimetry (ITC) experiments demonstrate that G5-BGG.has multiple molecular interactions with nucleic acids, which yield high.binding affinity toward nucleic acids. Interestingly, the in vitro transfection.efficiency and serum stability of G5-BGG are significantly improved when the.BGG modification ratio is just one. The integrated G5-BGG/DNA complex.is released from a PVA hydrogel sustainably, crosses the cell membrane and.escapes from endosome/lysosome. After local injection only once, these.features of the G5-BGG/DNA-loaded PVA hydrogel are found to improve antitumor.efficiency in vivo, and antitumor efficiency is significantly better than.PEI 25K. The results confirm that BGG is a potential group for developing.non-viral gene vectors with high transfection efficacy.
