Enzyme-catalysed assembly of DNA hydrogel
Enzyme-catalysed assembly of DNA hydrogel
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DOI：
10.1038/nmat1741
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发表时间：
2006-10-01
期刊：
影响因子：
41.2
通讯作者：
Luo, Dan
中科院分区：
文献类型：
--
作者：
Um, Soong Ho;Lee, Jong Bum;Luo, Dan
DNA是一种引人注目的聚合物，可以通过包括酶在内的大量分子工具进行操作（1）。人们已经构建了各种几何物体、周期阵列和纳米级器件（2-13）。以前，我们通过连接酶从分支DNA合成树状聚合物样DNA和DNA纳米条形码（14，15）。在这里，我们报告的水凝胶的建设完全从分支的DNA是三维的，可以交联的性质。这些DNA水凝胶具有生物相容性、生物可降解性、制造成本低且易于成型为所需的形状和尺寸。DNA水凝胶与其他生物启发的水凝胶（包括基于肽、基于藻酸盐和DNA（线性）-聚丙烯酰胺水凝胶（16-20））的明显区别在于交联通过有效的连接酶介导的反应实现。其优点在于，胶凝过程在生理条件下实现，并且包封在原位完成，包括蛋白质甚至活哺乳动物细胞的药物可以包封在液相中，从而消除了药物加载步骤并且还避免了变性条件。这些水凝胶的微调很容易通过调整分支DNA单体的初始浓度和类型来实现，从而允许水凝胶针对特定应用进行定制，例如受控药物递送、组织工程、3D细胞培养、细胞移植治疗和其他生物医学应用。
DNA is a remarkable polymer that can be manipulated by a large number of molecular tools including enzymes(1). A variety of geometric objects, periodic arrays and nanoscale devices have been constructed(2-13). Previously we synthesized dendrimer-like DNA and DNA nanobarcodes from branched DNA via ligases(14,15). Here we report the construction of a hydrogel entirely from branched DNA that are three-dimensional and can be crosslinked in nature. These DNA hydrogels were biocompatible, biodegradable, inexpensive to fabricate and easily moulded into desired shapes and sizes. The distinct difference of the DNA hydrogel to other bio-inspired hydrogels ( including peptide-based, alginate- based and DNA (linear)-polyacrylamide hydrogels(16-20)) is that the crosslinking is realized via efficient, ligase-mediated reactions. The advantage is that the gelling processes are achieved under physiological conditions and the encapsulations are accomplished in situ-drugs including proteins and even live mammalian cells can be encapsulated in the liquid phase eliminating the drug-loading step and also avoiding denaturing conditions. Fine tuning of these hydrogels is easily accomplished by adjusting the initial concentrations and types of branched DNA monomers, thus allowing the hydrogels to be tailored for specific applications such as controlled drug delivery, tissue engineering, 3D cell culture, cell transplant therapy and other biomedical applications.