Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis-induced resistance to Ralstonia solanacearum
Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis-induced resistance to Ralstonia solanacearum
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DOI：
10.1007/s00299-013-1515-1
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发表时间：
2013
影响因子：
6.2
通讯作者：
Hideki Takahashi;K. Nakaho;T. Ishihara;S. Ando;Takumi Wada;Y. Kanayama;S. Asano;S. Yoshida;S. Tsushima;M. Hyakumachi
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文献类型：
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作者：
Hideki Takahashi;K. Nakaho;T. Ishihara;S. Ando;Takumi Wada;Y. Kanayama;S. Asano;S. Yoshida;S. Tsushima;M. Hyakumachi
SA依赖信号通路的激活和JA依赖信号通路的抑制在B.苏云金杆菌诱导的抗病性。摘要苏云金芽孢杆菌（Bacillusthuringiensis）是一种高效的生物杀虫剂，作为一种潜在的植物病害生物防治剂，已引起人们的广泛关注。用过滤灭菌的B.苏云金杆菌通过系统激活植物防御系统来系统抑制由青枯菌引起的青枯病。对CF处理的番茄植株不同组织中抗病性标记P6基因表达的比较分析表明，CF处理的番茄植株中，B.苏云金杆菌诱导的防御系统在叶、茎和主根组织中被激活，而在侧根组织中不被激活。同时，R.在CF处理主根中，青枯病菌被显著抑制，但在CF处理侧根中没有。这种独特的激活防御反应和抑制R。青枯菌的主要和侧面组织的转录谱的差异反映在响应CF。在CF处理的主根，但没有CF处理的侧根，几个水杨酸（SA）响应防御相关基因的表达被特异性诱导，而茉莉酸（JA）相关基因的表达要么下调或不诱导响应CF。另一方面，编码乙烯（ET）相关蛋白的基因在主根和侧根组织中同样被诱导。因此，SA依赖性信号通路与ET依赖性信号通路的共激活和JA依赖性信号通路的抑制可能在B.苏云金杆菌诱导的抗病性。茄科植物番茄
Key messageActivation of SA-dependent signaling pathway and suppression of JA-dependent signaling pathway seem to play key roles inB. thuringiensis-induced resistance toR. solanacearumin tomato plants.AbstractBacillus thuringiensis, a well-known and effective bio-insecticide, has attracted considerable attention as a potential biological control agent for the suppression of plant diseases. Treatment of tomato roots with a filter-sterilized cell-free filtrate (CF) ofB. thuringiensissystemically suppresses bacterial wilt caused byRalstonia solanacearumthrough systemic activation of the plant defense system. Comparative analysis of the expression of thePathogenesis-Related 1(P6) gene, a marker for induced resistance to pathogens, in various tissues of tomato plants treated with CF on their roots suggested that theB. thuringiensis-induced defense system was activated in the leaf, stem, and main root tissues, but not in the lateral root tissue. At the same time, the growth ofR. solanacearumwas significantly suppressed in the CF-treated main roots but not in the CF-treated lateral roots. This distinct activation of the defense reaction and suppression ofR. solanacearumwere reflected by the differences in the transcriptional profiles of the main and lateral tissues in response to the CF. In CF-treated main roots, but not CF-treated lateral roots, the expression of several salicylic acid (SA)-responsive defense-related genes was specifically induced, whereas jasmonic acid (JA)-related gene expression was either down-regulated or not induced in response to the CF. On the other hand, genes encoding ethylene (ET)-related proteins were induced equally in both the main and lateral root tissues. Taken together, the co-activation of SA-dependent signaling pathway with ET-dependent signaling pathway and suppression of JA-dependent signaling pathway may play key roles inB. thuringiensis-induced resistance toR. solanacearumin tomato.