From anhydrobiosis to freeze-drying of eukaryotic cells
From anhydrobiosis to freeze-drying of eukaryotic cells
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DOI：
10.1016/s1095-6433(01)00505-0
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发表时间：
2002-03-01
影响因子：
2.3
通讯作者：
Crowe, JH
中科院分区：
文献类型：
--
作者：
Wolkers, WF;Tablin, F;Crowe, JH
利用从自然界学到的知识，已经有可能在干燥状态下稳定生物结构，包括完整的细胞。在干燥状态下的细胞或组织的稳定是相当大的实际意义，如在本审查中所述。在血库中，对干燥状态下的细胞的稳定化的需要是特别迫切的，其中血细胞（血小板和红细胞）的适当储存仍然是一个主要问题。人血小板在血库中储存5天，之后根据联邦法规将其丢弃。这种短暂的寿命导致了血小板的长期短缺。我们在这里报告，血小板可以通过与海藻糖一起冷冻干燥来保存，海藻糖是一种在生物体中发现的高浓度糖，可以自然地在干燥中存活。我们认为，这一发现将解决血小板的储存问题，并且这里建立的原则可以扩展到更复杂的真核细胞。(C)2002年爱思唯尔科技有限公司All rights reserved.
Using what has been learned from nature, it has become possible to stabilize biological structures, including intact cells, in the dry state. Stabilization of cells or tissues in the dried state is of considerable practical significance, as is described in this review. The need for stabilization of cells in the dried state is particularly urgent in bloodbanks, where proper storage of blood cells (platelets and erythrocytes) is still a major problem. Human blood platelets are stored in blood banks for 5 days, after which they are discarded according to Federal regulation. This short lifetime has led to a chronic shortage of platelets. We report here that platelets can be preserved by freeze-drying them with trehalose, a sugar Found at high concentrations in organisms that naturally survive drying. We suggest that this finding will obviate the storage problem with platelets and that the principles established here may be extended to more complex eukaryotic cells. (C) 2002 Elsevier Science Inc. All rights reserved.