A sensitive fluorimetric method for determination of platelet-bound and plasma free serotonin.
A sensitive fluorimetric method for determination of platelet-bound and plasma free serotonin.
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一种灵敏的荧光法，用于测定血小板结合和血浆游离血清素。
DOI：
10.1016/0009-8981(79)90213-4
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
G. L. Corona
中科院分区：
文献类型：
--
作者：
P. Frattini;M. Cucchi;G. Santagostino;G. L. Corona
本文介绍了一种灵敏、特异的荧光法测定血小板结合和血浆游离5-羟色胺的方法。将血清素浓缩并在Amberlite CG-50树脂上纯化，用0.15 mol/l HCl洗脱，并在295/350 nm处读取其天然荧光。良好的回收率（约90%）、重现性和灵敏度（最小可检测量为5 ng）使得该测定特别适用于血浆中游离5-羟色胺的检测。整个过程快速，一个工作日内可分析15-20个样品，适用于生物样品中5-羟色胺的常规测定。
A sensitive and specific fluorimetric assay for blood platelet-bound and plasma free serotonin is described. Serotonin is concentrated and purified on Amberlite CG-50 resin, eluted by 0.15 mol/1 HC1 and its native fluorescence is read at 295/350 nm. The good recovery (90% about), reproducibility and sensitivity (the smallest amount detectable is 5 ng) make the assay particularly useful for the detection of free serotonin in plasma. The whole procedure is rapid enough to permit the analysis of 15–20 samples within one working day and thus the method is suitable for routine determination of serotonin in biological specimens.