Rate of red blood cell destruction varies in different strains of mice infected with Plasmodium berghei-ANKA after chronic exposure.
Rate of red blood cell destruction varies in different strains of mice infected with Plasmodium berghei-ANKA after chronic exposure.
复制标题
慢性暴露后，红细胞破坏的速率在感染了伯氏疟原虫的不同小鼠的不同菌株中各不相同。
DOI：
10.1186/1475-2875-8-91
复制
发表时间：
2009-05-05
期刊：
影响因子：
3
通讯作者：
Hirayama K
中科院分区：
文献类型：
--
作者：
Helegbe GK;Huy NT;Yanagi T;Shuaibu MN;Yamazaki A;Kikuchi M;Yasunami M;Hirayama K
严重的疟疾贫血症的半免疫个人在全流行区已被观察到发生在低寄生虫密度与个别差异的反应。因此，以下被认为涉及：未感染的红细胞破坏的自身免疫介导的机制，以及宿主遗传因素，以解释在相同的疟疾传播下个体反应的差异。在这项研究中，红细胞（RBC）的破坏程度在不同品系的半免疫小鼠模型在相对较低的寄生虫血症进行了研究。为了产生半免疫力，四种小鼠经过几个周期的感染和治疗。用免疫荧光法和ELISA法检测血清中抗红细胞自身抗体，并研究其与血液学指标和寄生虫血症的关系。在产生半免疫状态后用伯氏疟原虫ANKA攻击，观察到不同品系小鼠的血红蛋白（Hb）平均百分比下降（Balb/c = 47.1%，NZW = 30.05%，C57BL/6 = 28.44%，CBA = 25.1%），各菌株的突变发生在不同的日期（对于Balb/c，平均周期= 13.6天;对于C57 BL/6、NZW和CBA，平均周期分别= 10.6、10.8、10.9天）。免疫荧光法观察到抗体与四种半免疫小鼠血清中的白色血影红细胞结合。Balb/c（73.6）中每次寄生虫血症的平均Hb下降百分比最高，其次是C57 BL/6（8.6）、CBA（6.9）和NZW（4.0），p = 0.0005。因此，血影红细胞的自身抗体水平与贫血程度相关，与其他菌株相比，Balb/c中的抗体水平最高，p <0.001。本研究中呈现的结果似乎表明，抗RBC自身抗体可能参与在相对低寄生虫负荷下半免疫小鼠中未感染RBC的破坏。宿主遗传因素也可能影响自身免疫介导的RBC破坏的结果，这是由于每%寄生虫血症的Hb损失的变化和每个半免疫小鼠品系的抗体滴度的差异。然而，应该在分子水平上进行进一步的研究来证实这一点。
Severe malaria anaemia in the semi-immune individuals in the holo-endemic area has been observed to occur at low parasite density with individual variation in the responses. Thus the following has been thought to be involved: auto-immune-mediated mechanisms of uninfected red blood cell destruction, and host genetic factors to explain the differences in individual responses under the same malaria transmission. In this study, the extent of red blood cell (RBC) destruction in different strains of semi-immune mice model at relatively low parasitaemia was studied. To generate semi-immunity, four strains of mice were taken through several cycles of infection and treatment. By means of immunofluorescent assay and ELISA, sera were screened for anti-erythrocyte auto-antibodies, and their relationship with haematological parameters and parasitaemia in the strains of semi-immune mice was investigated. Upon challenge with Plasmodium berghei ANKA after generating semi-immune status, different mean percentage haemoglobin (Hb) drop was observed in the mice strains (Balb/c = 47.1%; NZW = 30.05%; C57BL/6 = 28.44%; CBA = 25.1%), which occurred on different days for each strain (for Balb/c, mean period = 13.6 days; for C57BL/6, NZW, and CBA mean period = 10.6, 10.8, 10.9 days respectively). Binding of antibody to white ghost RBCs was observed in sera of the four strains of semi-immune mice by immunofluorescence. Mean percentage Hb drop per parasitaemia was highest in Balb/c (73.6), followed by C57BL/6 (8.6), CBA (6.9) and NZW (4.0), p = 0.0005. Consequently, auto-antibodies level to ghost RBC were correlated with degree of anaemia and were highest in Balb/c, when compared with the other strains, p < 0.001. The results presented in this study seem to indicate that anti-RBC auto-antibodies may be involved in the destruction of uninfected RBC in semi-immune mice at relatively low parasite burden. Host genetic factors may also influence the outcome of auto-immune mediated destruction of RBC due to the variation in Hb loss per % parasitaemia and differences in antibody titer for each semi-immune mice strain. However, further studies at the molecular level ought to be carried out to confirm this.
登录
查看更多内容
影响因子：
2.7
作者：
Huy, NT;Serada, S;Kamei, K
通讯作者：
Kamei, K
影响因子：
39.2
作者：
ADNER, MM;ALTSTATT, LB;CONRAD, ME
通讯作者：
CONRAD, ME
影响因子：
3
作者：
Lamb, Tracey J.;Langhorne, Jean
通讯作者：
Langhorne, Jean
影响因子：
1.8
作者：
Goes, T. S.;Goes, V. S.;Gontijo, C. M.
通讯作者：
Gontijo, C. M.
影响因子：
20.3
作者：
Evans, KJ;Hansen, DS;Schofield, L
通讯作者：
Schofield, L