The origin of chondrocytes in the nucleus pulposus and histologic findings associated with the transition of a notochordal nucleus pulposus to a fibrocartilaginous nucleus pulposus in intact rabbit intervertebral discs
The origin of chondrocytes in the nucleus pulposus and histologic findings associated with the transition of a notochordal nucleus pulposus to a fibrocartilaginous nucleus pulposus in intact rabbit intervertebral discs
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DOI：
10.1097/00007632-200305150-00005
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发表时间：
2003-05-15
期刊：
影响因子：
3
通讯作者：
An, HS
中科院分区：
文献类型：
--
作者：
Kim, KW;Lim, TH;An, HS
研究设计。对完整的兔腰椎间盘进行组织学检查。目的。证明髓核中软骨细胞的起源，并记录与脊索向纤维软骨髓核转变相关的组织学发现。背景数据摘要。人类髓核经历从脊索到纤维软骨髓核的时间转变。然而，髓核中软骨细胞形成纤维软骨的起源和转变机制仍不清楚。方法。用光学和偏光显微镜观察从 125 个完整兔椎间盘获得的苏木精-伊红和番红 O 染色的载玻片。结果。在检查的 125 个椎间盘中，58 个有脊索髓核。剩余的椎间盘具有带有纤维软骨板或纤维软骨纤维的髓核。髓核中发现的所有形式的纤维软骨板层和纤维都是由源自软骨终板并从软骨终板迁移的软骨细胞形成的。软骨细胞的起源沿向心方向从软骨终板的外围向中心进行。因此，新形成的纤维软骨板层和纤维最初涉及髓核外围区域的替换，随后是中央区域的替换。这种向心顺序置换机制减小了脊索组织的尺寸，同时增加了椎间盘的板层结构。结论。完整兔髓核中的软骨细胞起源于软骨终板并从软骨终板迁移。软骨细胞通过沉积方向将脊索髓核转变为纤维软骨髓核。
Study Design. Intact rabbit lumbar intervertebral discs were examined histologically.Objectives. To demonstrate the origin of chondrocytes in the nucleus pulposus, and to document histologic findings associated with the transition of a notochordal to a fibrocartilaginous nucleus pulposus.Summary of Background Data. A human nucleus pulposus undergoes a chronological transition from a notochordal to a fibrocartilaginous nucleus pulposus. However, the origin of chondrocytes forming fibrocartilage in the nucleus pulposus and the mechanisms of transition remain unknown.Methods. Hematoxylin-eosin- and safranin O-stained slides obtained from 125 intact rabbit intervertebral discs were observed with light and polarized light microscopy.Results. Of the 125 intervertebral discs examined, 58 had a notochordal nucleus pulposus. The remaining intervertebral discs had a nucleus pulposus with fibrocartilage lamellas or fibrocartilage fibers. All forms of fibrocartilage lamellas and fibers found in the nucleus pulposus were formed by chondrocytes that had originated and migrated from the cartilage endplate. The origin of chondrocytes proceeded in a centripetal direction from the periphery toward the center of the cartilage endplate. The newly formed fibrocartilage lamellas and fibers, therefore, initially involved replacement of the peripheral regions of the nucleus pulposus, followed by replacement of the central region. This centripetal sequential replacement mechanism decreased the size of the notochordal tissue while increasing the lamellar structure of the intervertebral disc.Conclusions. Chondrocytes in the intact rabbit nucleus pulposus originated and migrated from the cartilage endplate. The chondrocytes changed notochordal nucleus pulposus into fibrocartilaginous nucleus pulposus by depositing direction.