Thin layer chromatography/plasma assisted multiwavelength laser desorption ionization mass spectrometry for facile separation and selective identification of low molecular weight compounds.
Thin layer chromatography/plasma assisted multiwavelength laser desorption ionization mass spectrometry for facile separation and selective identification of low molecular weight compounds.
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DOI：
10.1021/ac202732y
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发表时间：
2012-01
影响因子：
7.4
通讯作者：
Jialing Zhang;Zhigui Zhou;Jian-Wang Yang;Wei Zhang;Yu Bai;Huwei Liu
中科院分区：
文献类型：
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作者：
Jialing Zhang;Zhigui Zhou;Jian-Wang Yang;Wei Zhang;Yu Bai;Huwei Liu
构建了一种新型等离子体辅助多波长（1064、532 和 355 nm）激光解吸电离质谱（PAMLDI-MS）系统，并通过与薄层色谱（TLC）相结合应用于低分子量化合物的分析。 TLC/PAMLDI-MS系统成功地集成了TLC、多波长激光烧蚀和实时直接分析（DART）激发态等离子体，并被证明可以有效地轻松分离和选择性鉴定低分子量化合物。利用自动化三维平台来促进分析程序，系统优化了 TLC/PAMLDI-MS 的所有参数，并讨论了解吸/电离机制。三波长激光与基于 DART 的系统的成功结合扩大了分析物的范围，并为 TLC 中化合物的解吸提供了广阔的可能性。实验结果清楚地表明激光解吸与波长相关。 PAMLDI-MS系统成功应用于正相硅胶上分离的不同种类样品中低分子量化合物的检测，例如染料混合物、药物标准品和茶叶提取物，检测水平为5 ng/mm(2)。
A novel plasma assisted multiwavelength (1064, 532, and 355 nm) laser desorption ionization mass spectrometry (PAMLDI-MS) system was fabricated and applied in the analysis of low molecular weight compounds through combination with thin layer chromatography (TLC). The TLC/PAMLDI-MS system successfully integrated TLC, the multiwavelength laser ablation, and the excitated state plasma from direct analysis in real time (DART) and was proved to be effective in the facile separation and selective identification of low molecular weight compounds. An automated three-dimensional platform was utilized to facilitate the analysis procedures with all the parameters of the TLC/PAMLDI-MS systematically optimized, and the desorption/ionization mechanisms were discussed. The successful combination of three-wavelength laser with DART based system extended the range of the analytes and provided broad possibilities for the compound desorption from the TLC. The experimental results clearly showed that the laser desorption was wavelength dependent. The PAMLDI-MS system was successfully applied in the detection of low molecular weight compounds from different kinds of samples separated on a normal-phase silica gel, such as dye mixtures, drug standards, and tea extract, with the detection level of 5 ng/mm(2).