Dynamic changes in the monolayer permeability and rearrangement microfilaments in ECV304 cells after Dengue virus infection
Dynamic changes in the monolayer permeability and rearrangement microfilaments in ECV304 cells after Dengue virus infection
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发表时间：
2005
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通讯作者：
An Jing
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作者：
An Jing
为研究登革病毒感染后内皮样细胞微丝的实时重排和单层通透性的变化，应用免疫荧光法观察了ECV304细胞和人脐静脉内皮细胞（HUVEC）微丝的重排，用transwell模型观察病毒对ECV304细胞单层通透性的影响，用空斑法测定病毒滴度，结果表明，病毒对ECV304细胞单层通透性的影响与病毒对ECV304细胞单层通透性的影响有关在登革病毒感染后1小时，在HUVEC和ECV304细胞中均可以证明微丝的重排，细胞膜通透性增加的高峰出现在感染后第3天，与病毒滴度的高峰和细胞膜应力纤维的急剧减少相一致，提示细胞膜通透性的增加可能与病毒感染后引起的微丝重排有关。这些细胞中的高滴度病毒。
To investigate the real-time rearrangement of microfilaments andalteration in the monolayer permeability in endothelium-like cells five days after Dengue virus infection,immunofluorescence assay was applied to observe the rearrangement of microfilaments in ECV304 cells and human umbilical vein endothelial cells(HUVEC),and the transwell model was used to investigate the alteration of the monolayer permeability in ECV304 cells.The virus titer was measured by plaque assay.It was found that rearrangement of the microfilaments could be demonstrated both in HUVEC and ECV304 cells one hour after Dengue virus infection,and peak of increased monolayer permeability occurred at the third day after infection in consistent with the top of virus titer and the dramatic decrease of the stress fibers.It suggests that the increase in monolayer permeability in ECV304 cells might be associated with the rearrangement of microfilaments induced by the high titered virus in these cells.