The role of fluctuations in tRNA selection by the ribosome
The role of fluctuations in tRNA selection by the ribosome
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DOI：
10.1073/pnas.0705988104
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发表时间：
2007-08-21
影响因子：
11.1
通讯作者：
Chu, Steven
中科院分区：
文献类型：
--
作者：
Lee, Tae-Hee;Blanchard, Scott C.;Chu, Steven
核糖体如何以异常高的准确度解码蛋白质序列信息的详细机制，经过40年的研究，仍然难以捉摸。选择正确的转运RNA（tRNA）转移正确的氨基酸的一个关键因素是核糖体和tRNA之间的“诱导配合”。通过使用单分子的方法，诱导适合机制被证明是有利的位置后，初始密码子识别正确的tRNA。我们提供的证据表明，这种定位和热波动的差异构成了tRNA初始选择的主要机制。这项工作表明，热波动发挥了关键作用的底物选择的酶。
The detailed mechanism of how the ribosome decodes protein sequence information with an abnormally high accuracy, after 40 years of study, remains elusive. A critical element in selecting correct transfer RNA (tRNA) transferring correct amino acid is '' induced fit '' between the ribosome and tRNA. By using single-molecule methods, the induced fit mechanism is shown to position favorably the correct tRNA after initial codon recognition. We provide evidence that this difference in positioning and thermal fluctuations constitutes the primary mechanism for the initial selection of tRNA. This work demonstrates thermal fluctuations playing a critical role in the substrate selection by an enzyme.