In Vivo Activity of Hydrogen-Peroxide Generating Electrochemical Bandage Against Murine Wound Infections.
In Vivo Activity of Hydrogen-Peroxide Generating Electrochemical Bandage Against Murine Wound Infections.
复制标题
产生过氧化氢的电化学绷带对抗小鼠伤口感染的体内活性。
DOI：
10.1002/adtp.202300059
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发表时间：
2023
影响因子：
4.6
通讯作者：
Patel,Robin
中科院分区：
文献类型：
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作者：
Raval,YashS;Fleming,Derek;Mohamed,Abdelrhman;Karau,MelissaJ;Mandrekar,JayawantN;Schuetz,AudreyN;GreenwoodQuaintance,KerrylE;Beyenal,Haluk;Patel,Robin
由伤口床中的抗生素抗性细菌形成的生物膜挑战伤口感染的治疗。在这项工作中，一种新型的电化学绷带（e-绷带）由碳纤维织物组成，并由可穿戴的恒电位仪控制，旨在持续提供少量的过氧化氢（H2 O2），对耐甲氧西林金黄色葡萄球菌（MRSA），多重耐药铜绿假单胞菌（MDR-PA）和混合物种（MRSA和MDR-PA）伤口感染进行了评估。用上述细菌感染在Swiss韦伯斯特小鼠上产生的伤口，并使生物膜在伤口床上形成3天。e-绷带在-0.6 VAg/AgCl下极化时可将溶解氧电化学还原为H2 O2，将其置于感染的伤口床上并连续极化48 h。极化e-绷带治疗导致单种和混合种伤口感染显著减少（p<0.001）。电子绷带处理后，电子显微镜显示细菌细胞降解，组织病理学显示炎症宿主反应无明显变化。血液生化检查没有异常。总而言之，这项工作的结果表明，所描述的产生H2 O2的电子绷带可以减少体内MRSA、MDR-PA和混合物种伤口生物膜，并且应该进一步开发作为治疗伤口感染的潜在无抗生素策略。
Biofilms formed by antibiotic‐resistant bacteria in wound beds challenge the treatment of wound infections. In this work, the activity of a novel electrochemical bandage (e‐bandage) composed of carbon fabric and controlled by a wearable potentiostat, designed to continuously deliver low amounts of hydrogen peroxide (H2O2), is evaluated against methicillin‐resistantStaphylococcus aureus(MRSA), multidrug‐resistantPseudomonas aeruginosa(MDR‐PA) and mixed‐species (MRSA and MDR‐PA) wound infections. Wounds created on Swiss Webster mice are infected with the above‐named bacteria and biofilms allow to establish in wound beds for 3 days. e‐Bandages, which electrochemically reduce dissolved oxygen to H2O2when polarized at −0.6 VAg/AgCl, are placed atop infected wound beds and polarized continuously for 48 h. Polarized e‐bandage treatment results in significant reductions (p<0.001) of both monospecies and mixed‐species wound infections. After e‐bandage treatment, electron microscopy shows degradation of bacterial cells, and histopathology shows no obvious alterations to the inflammatory host response. Blood biochemistries show no abnormalities. Taken all together, results of this work suggest that the described H2O2‐producing e‐bandage can reduce in vivo MRSA, MDR‐PA, and mixed‐species wound biofilms, and should be further developed as a potential antibiotic‐free strategy for treatment of wound infections.