Large-Scale Expression and Purification of Mumps Virus Hemagglutinin-Neuraminidase for Structural Analyses and Glycan-Binding Assays
Large-Scale Expression and Purification of Mumps Virus Hemagglutinin-Neuraminidase for Structural Analyses and Glycan-Binding Assays
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DOI：
10.1007/978-1-0716-0430-4_55
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发表时间：
2020-01-01
期刊：
影响因子：
--
通讯作者：
Hashiguchi, Takao
中科院分区：
文献类型：
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作者：
Kubota, Marie;Hashiguchi, Takao
许多病毒利用细胞表面聚糖作为受体进入宿主细胞。病毒表面糖蛋白特异性地与聚糖基序相互作用，这强烈地促进了病毒的趋向性。近年来，通过测定宿主细胞聚糖受体与腮腺炎病毒（MuV）血凝素-神经氨酸酶（MuV- hn）蛋白复合物的共晶结构，研究了其与宿主细胞聚糖受体之间的相互作用。在这里，我们描述了大规模表达、纯化和结晶MuV-HN蛋白的方案，用于结构分析和聚糖结合分析，其总体目标是研究聚糖-蛋白相互作用。
Many viruses utilize cell-surface glycans as receptors for host cell entry. Viral surface glycoproteins specifically interact with glycan motifs, which strongly contributes to viral tropism. Recently, the interactions between host cell glycan receptors and the mumps virus (MuV) hemagglutinin-neuraminidase (MuV-HN) protein were characterized by determining the co-crystal structure of MuV-HN in complex with glycan receptors. Here, we describe protocols for large-scale expression, purification and crystallization of MuV-HN proteins for structural analyses and glycan-binding assays with the overarching goal of investigating glycan-protein interactions.