A phylogenetically conserved APETALA2/ETHYLENE RESPONSE FACTOR, ERF12, regulates Arabidopsis floral development
A phylogenetically conserved APETALA2/ETHYLENE RESPONSE FACTOR, ERF12, regulates Arabidopsis floral development
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DOI：
10.1007/s11103-019-00936-5
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发表时间：
2019-12-05
影响因子：
5.1
通讯作者：
Werr, W.
中科院分区：
文献类型：
--
作者：
Chandler, J. W.;Werr, W.
拟南芥乙烯反应因子12（ERF 12），水稻MULTIFLORET SPIKELET 1同源基因，多效性地影响分生组织的特性，花的叶序和器官的起始，并在被子植物中保守。生殖发育需要通过正、负调节子和网络整合子协调调节分生组织的身份和成熟以及侧生器官的启动。我们已经鉴定了乙烯应答因子12（ERF 12）作为拟南芥中的多花小穗1（MFS 1）的直向同源物。ERF 12功能的丧失会多效性地影响生殖发育，包括在第一次形成的花中不完全萌发时的有缺陷的花叶序和增加的花器官分生，特别是多余的萼片。在早期形成的花野生型花器官的数量是不稳定的，表明花分生组织成熟涉及器官发生的位置信息的稳定，以及适当的身份。ERF 12表型的一个子集部分定义了一个狭窄的发育时间窗口，表明ERF 12的功能异时微调野生型花数的随机变化，类似于MFS 1，促进分生组织的身份。ERF 12的表达包围在花序分生组织外围的初期花原基，并且在整个花分生组织和萼片间区域都很强。ERF 12是一种假定的转录抑制因子，在遗传上与其亲属DORNRoSCHEN、DORNRoSCHEN-LIKE和PUCHI的功能相反，并与APETALA 2通路会聚。系统发育分析表明，ERF 12在所有真双子叶植物中是保守的，并且在被子植物进化中伴随着花多样性的产生而出现。
Key message Arabidopsis ETHYLENE RESPONSE FACTOR12 (ERF12), the rice MULTIFLORET SPIKELET1 orthologue pleiotropically affects meristem identity, floral phyllotaxy and organ initiation and is conserved among angiosperms. Reproductive development necessitates the coordinated regulation of meristem identity and maturation and lateral organ initiation via positive and negative regulators and network integrators. We have identified ETHYLENE RESPONSE FACTOR12 (ERF12) as the Arabidopsis orthologue of MULTIFLORET SPIKELET1 (MFS1) in rice. Loss of ERF12 function pleiotropically affects reproductive development, including defective floral phyllotaxy and increased floral organ merosity, especially supernumerary sepals, at incomplete penetrance in the first-formed flowers. Wildtype floral organ number in early formed flowers is labile, demonstrating that floral meristem maturation involves the stabilisation of positional information for organogenesis, as well as appropriate identity. A subset of erf12 phenotypes partly defines a narrow developmental time window, suggesting that ERF12 functions heterochronically to fine-tune stochastic variation in wild type floral number and similar to MFS1, promotes meristem identity. ERF12 expression encircles incipient floral primordia in the inflorescence meristem periphery and is strong throughout the floral meristem and intersepal regions. ERF12 is a putative transcriptional repressor and genetically opposes the function of its relatives DORNRoSCHEN, DORNRoSCHEN-LIKE and PUCHI and converges with the APETALA2 pathway. Phylogenetic analysis suggests that ERF12 is conserved among all eudicots and appeared in angiosperm evolution concomitant with the generation of floral diversity.