Bio-hydrogen production by mixed culture of photo- and dark-fermentation bacteria
Bio-hydrogen production by mixed culture of photo- and dark-fermentation bacteria
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DOI：
10.1016/j.ijhydene.2009.05.005
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发表时间：
2010-04-01
影响因子：
7.2
通讯作者：
Xie, Guo-Jun
中科院分区：
文献类型：
--
作者：
Liu, Bing-Feng;Ren, Nan-Qi;Xie, Guo-Jun
利用酪酸梭菌和粪红假单胞菌RLD-53在葡萄糖为唯一底物的混合培养条件下产氢。由于光发酵细菌和暗发酵细菌在生长速度和耐酸能力上存在较大差异，本文对这两种细菌不同比例的直接混合培养进行了研究。对不同时期的产氢量、挥发酸、pH和生物量进行了评价。丁酸假单胞菌产生的乙酸和丁酸是最终发酵产物，是光发酵细菌的有效底物。合作是以一种类似食物链的方式形成的。但与丁酸杆菌产生的挥发酸相比，光发酵细菌对挥发酸的利用率要低得多。结果表明，当pH值急剧下降时，光发酵细菌的生长受到限制。丁酸杆菌与粪链球菌RLD-53的最佳比例为1：600。最大产氢量为122.4 ml·H~(-2)/罐，产氢速率为0.5ml·H~(-2)/ml/d。(C)2009年，T.Nejat Veziroglu教授。爱思唯尔有限公司出版。保留所有权利。
Clostridium butyricum and Rhodopseudomonas faecalis RLD-53 were employed to produce hydrogen in mixed culture with glucose as sole substrate. Due to the great difference on growth rate and acid-resistant capacity between photo-fermentative bacteria and dark-fermentative bacteria, directly mixed culture of the two kinds of bacteria in different ratio was studied in this work. Hydrogen yield, volatile acids, pH and biomass in different periods were evaluated. Acetic acid and butyric acid produced by C. butyricum were dominant terminal fermentation products, and they were effective substrates for photo-fermentative bacteria. The cooperation was formed in a way like food chain. But compared to the production rate of volatile acids produced by C. butyricum, the utilization rate by photo-fermentative bacteria was far slower. The results demonstrated that the growth of photo-fermentative bacteria was limited when pH decreased sharply. The best ratio of C. butyricum to R. faecalis RLD-53 was 1:600. The maximum yield of hydrogen reached 122.4 ml-H-2/vessel and hydrogen production rate was 0.5 ml-H-2/ml-culture/day. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.