A genome-wide analysis in Saccharomyces cerevisiae demonstrates the influence of chromatin modifiers on transcription
A genome-wide analysis in Saccharomyces cerevisiae demonstrates the influence of chromatin modifiers on transcription
复制标题
DOI：
10.1038/ng1965
复制
发表时间：
2007-03-01
期刊：
影响因子：
30.8
通讯作者：
Kupiec, Martin
中科院分区：
文献类型：
--
作者：
Steinfeld, Israel;Shamir, Ron;Kupiec, Martin
染色质结构在转录调控中很重要。许多影响染色质结构的因素已被确定，但它们参与的转录程序仍知之甚少。染色质修饰剂与 DNA 结合转录因子一起参与转录控制。高通量实验方法允许在全基因组范围内鉴定转录因子的结合位点以及在各种环境和遗传条件下定量基因表达。我们开发了一种新方法，使用大量可用数据来剖析染色质结构对转录的贡献。我们测量并表征转录因子功能对特定染色质修饰剂的依赖性。我们将我们的方法应用于酿酒酵母，使用了来自 26 项不同研究的染色质修饰缺陷菌株的 170 个基因表达谱概要。我们的方法成功地识别了染色质修饰剂和转录因子之间已知的复杂遗传相互作用，并揭示了许多以前未知的遗传相互作用，首次给出了染色质结构对真核生物转录的贡献的全基因组图景。
Chromatin structure is important in transcription regulation. Many factors influencing chromatin structure have been identified, but the transcriptional programs in which they participate are still poorly understood. Chromatin modifiers participate in transcriptional control together with DNA-bound transcription factors. High-throughput experimental methods allow the genome-wide identification of binding sites for transcription factors as well as quantification of gene expression under various environmental and genetic conditions. We have developed a new methodology that uses the vast amount of available data to dissect the contribution of chromatin structure to transcription. We measure and characterize the dependence of transcription factor function on specific chromatin modifiers. We apply our methodology to S. cerevisiae, using a compendium of 170 gene expression profiles of strains defective for chromatin modifiers, taken from 26 different studies. Our method succeeds in identifying known intricate genetic interactions between chromatin modifiers and transcription factors and uncovers many previously unknown genetic interactions, giving the first genome-wide picture of the contribution of chromatin structure to transcription in a eukaryote.