The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice
The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice
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DOI：
10.3791/53319
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发表时间：
2015-11-01
影响因子：
1.2
通讯作者：
Janssen, Edith M.
中科院分区：
文献类型：
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作者：
Klarquist, Jared;Janssen, Edith M.
系统性红斑狼疮（SLE）是一种自身免疫性疾病，具有多种临床和免疫学表现。几种自发和诱导动物模型反映了人类疾病的常见组成部分，包括 bm12 转移模型。在转移 bm12 脾细胞或纯化的 CD4 T 细胞后，C57BL/6 小鼠迅速产生大量滤泡辅助 T 细胞 (Tfh)、生发中心 (GC) B 细胞和浆细胞，随后产生高水平的循环抗核抗体。由于该模型利用纯 C57BL/6 背景的小鼠，研究人员可以在相对较短的时间内快速、轻松地研究转基因或基因敲除小鼠品系的疾病进展。在这里，我们描述了通过多色流式细胞术诱导模型和定量 Tfh、GC B 细胞和浆细胞的方案。重要的是，这些方案还可用于表征大多数 SLE 小鼠模型的疾病特征，并识别其他疾病模型中的 Tfh、GC B 细胞和浆细胞。
Systemic lupus erythematosus (SLE) is an autoimmune disease with diverse clinical and immunological manifestations. Several spontaneous and inducible animal models mirror common components of human disease, including the bm12 transfer model. Upon transfer of bm12 splenocytes or purified CD4 T cells, C57BL/6 mice rapidly develop large frequencies of T follicular helper cells (Tfh), germinal center (GC) B cells, and plasma cells followed by high levels of circulating anti-nuclear antibodies. Since this model utilizes mice on a pure C57BL/6 background, researchers can quickly and easily study disease progression in transgenic or knockout mouse strains in a relatively short period of time. Here we describe protocols for the induction of the model and the quantitation Tfh, GC B cells, and plasma cells by multi-color flow cytometry. Importantly, these protocols can also be used to characterize disease in most mouse models of SLE and identify Tfh, GC B cells, and plasma cells in other disease models.