Introducing multiple bio-functional groups on the poly(ether sulfone) membrane substrate to fabricate an effective antithrombotic bio-interface
Introducing multiple bio-functional groups on the poly(ether sulfone) membrane substrate to fabricate an effective antithrombotic bio-interface
复制标题
在聚醚砜膜基底上引入多个生物官能团构建有效的抗血栓生物界面
DOI：
10.1039/c7bm00673j
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发表时间：
2017
影响因子：
6.6
通讯作者：
Zhao Changsheng
中科院分区：
文献类型：
--
作者：
Wang Lingren;He Min;Gong Tao;Zhang Xiang;Zhang Lincai;Liu Tao;Ye Wei;Pan Changjiang;Zhao Changsheng
生物材料表面的功能基团对血液相容性起着重要的作用。构建一种有效的抗血栓生物界面在聚醚砜（PES）膜表面上，分三步将羧酸钠（-COONa）、磺酸钠（-SO 3 Na）和氨基（-NH 2）基团的生物官能团引入到PES膜表面上：含羧基（-COOH）的PES（CPES）和含磺酸钠（-SO 3 Na）和氨基（-NH 2）的水溶性PES（SNPES）的合成;通过CPES与PES共混，在PES膜上引入羧基，通过氨基和羧基的偶联，将SNPES接枝到CPES/PES膜上。其理化性质和生物活性取决于添加剂的比例。引入生物功能基团后，通过抑制血小板粘附和活化，延长凝血时间，抑制血液相关补体和白细胞相关补体受体激活，证实了改性膜的优良血液相容性。此外，细胞试验表明，改性膜显示出更好的细胞相容性，在内皮细胞增殖比原始PES膜由于协同促进的功能基团。总之，这些结果表明，改性膜在使用血液接触材料的领域，如血液透析和表面内皮化，具有很大的潜力。
It has been widely recognized that functional groups on biomaterial surfaces play important roles in blood compatibility. To construct an effective antithrombotic bio-interface onto the poly(ether sulfone) (PES) membrane surface, bio-functional groups of sodium carboxylic (–COONa), sodium sulfonic (–SO3Na) and amino (–NH2) groups were introduced onto the PES membrane surface in three steps: the synthesis of PES with carboxylic (–COOH) groups (CPES) and water-soluble PES with sodium sulfonic (–SO3Na) groups and amino (–NH2) groups (SNPES); the introduction of carboxylic groups onto the PES membrane by blending CPES with PES; and the grafting of SNPES onto CPES/PES membranes via the coupling of amino groups and carboxyl groups. The physical/chemical properties and bioactivities were dependent on the proportions of the additives. After introducing bio-functional groups, the excellent hemocompatibility of the modified membranes was confirmed by the inhibited platelet adhesion and activation, prolonged clotting times, suppressed blood-related complement and leukocyte-related complement receptor activations. Furthermore, cell tests indicated that the modified membranes showed better cytocompatibility in endothelial cell proliferation than the pristine PES membrane due to the synergistic promotion of the functional groups. To sum up, these results suggested that modified membranes present great potential in fields using blood-contacting materials, such as hemodialysis and surface endothelialization.