Sequential 1H-NMR assignment and solution structure of bovine pancreatic ribonuclease A.
Sequential 1H-NMR assignment and solution structure of bovine pancreatic ribonuclease A.
复制标题
牛胰腺核糖核酸酶 A 的连续 1H-NMR 归属和溶液结构。
DOI：
10.1111/j.1432-1033.1989.tb21092.x
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
J. Herranz
中科院分区：
文献类型：
--
作者：
M. Rico;M. Bruix;J. Santoro;Carlos A. González;José L. Neira;J. Nieto;J. Herranz
用序列特异性方法测定了牛胰核糖核酸酶（RNase A）大部分残基的~ 1H-NMR谱。自旋系统的识别和分类已进行了二维相敏相关光谱（360 MHz）和单中继相干转移光谱。序列特异性的分配已通过相敏二维核奥弗豪泽效应光谱实现。为了克服光谱重叠的问题，已经使用了（a）部分交换的RNA酶A的详尽分析（D2 O中的光谱），（B）与枯草杆菌蛋白酶修饰的酶（RNA酶S）的比较和（c）由pH和温度的变化引起的小的光谱扰动。二级结构元素已被确定从所观察到的连续，中期和长期的核Overhauser效应与数据从酰胺交换率。收集的所有信息得出的结论是，晶体和溶液结构非常相似。
Assignments for 1H-NMR resonances of most of the residues of bovine pancreatic ribonuclease (RNase A) have been obtained by sequence-specific methods. Identification and classification of spin systems have been carried out by two-dimensional phase-sensitive correlated spectroscopy (360 MHz) and single relayed coherence transfer spectroscopy. Sequence-specific assignments have been achieved by phase-sensitive two-dimensional nuclear Overhauser effect spectroscopy. To overcome the problem of spectral overlap use has been made of (a) an exhaustive analysis of partly exchanged RNase A (spectra in D2O), (b) a comparison with the subtilisin-modified enzyme (RNase S) and (c) small spectral perturbations caused by changes in pH and temperature. The secondary structure elements have been identified from the observed sequential, medium and long-range nuclear Overhauser effects together with data from amide-exchange rates. All information collected leads to the conclusion that the crystal and the solution structures are closely similar.
影响因子：
2.9
作者：
LYNN, RM;KONISHI, Y;SCHERAGA, HA
通讯作者：
SCHERAGA, HA