Di-4-ANEPPDHQ, a fluorescent probe for the visualisation of membrane microdomains in living Arabidopsis thaliana cells
Di-4-ANEPPDHQ, a fluorescent probe for the visualisation of membrane microdomains in living Arabidopsis thaliana cells
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Di-4-ANEPPDHQ，一种用于活拟南芥细胞膜微域可视化的荧光探针
DOI：
10.1016/j.plaphy.2014.12.015
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发表时间：
2015-02-01
影响因子：
6.5
通讯作者：
Wan, Yinglang
中科院分区：
文献类型：
--
作者：
Zhao, Xiaoyu;Li, Ruili;Wan, Yinglang
富含胆固醇的微区，也称为脂筏，是具有高度结构有序性的纳米级膜结构。由于这些微区在动态细胞学事件中起着重要作用，如细胞信号传导和膜运输，因此检测和跟踪微区行为对现代膜生理学的研究至关重要。目前，微区的观察主要是基于使用人工交联荧光探针检测特定的筏居民成分。然而，只有少数微区特异性荧光染料可用于植物细胞生物学研究。在这项研究中，二-4-ANEPPDHQ的物理性质进行了分析。使用共聚焦激光扫描显微镜（CLSM）为基础的方法在可视化的拟南芥活细胞中的微域进行了评估。结果证实，广义极化（GP）方法可用于定量可视化活植物细胞中的膜顺序。发现该染料在植物根表皮细胞和根毛中具有低细胞毒性。这些研究结果表明，di 4-ANEPPDHQ是一个合适的工具，活植物细胞中的微域的可视化。(C)2014年Elsevier Masson SAS。All rights reserved.
Cholesterol-enriched microdomains, also called lipid rafts, are nanoscale membrane structures with a high degree of structural order. Since these microdomains play important roles in dynamic cytological events, such as cell signalling and membrane trafficking, the detection and tracking of microdomain behaviours are crucial to studies on modern membrane physiology. Currently, observation of microdomains is mostly based on the detection of specific raft-resident constituents using artificial cross-link fluorescent probes. However, only a few microdomain-specific fluorescent dyes are available for plant cell biology studies. In this study, the photophysical properties of di-4-ANEPPDHQ were analysed. The use of confocal laser scanning microscope (CLSM)-based methods in the visualisation of microdomains in living cells of Arabidopsis thaliana was assessed. The results confirmed that the generalised polarisation (GP) method can be used to quantitatively visualise the membrane orders in live plant cells. This dye was found to have low cytotoxicity in plant root epidermal cells and root hairs. These findings suggest that di4-ANEPPDHQ is an appropriate tool for the visualisation of microdomains in living plant cells. (C) 2014 Elsevier Masson SAS. All rights reserved.