Ratiometric Photoacoustic Nanoprobe for Bioimaging of Cu2+
Ratiometric Photoacoustic Nanoprobe for Bioimaging of Cu2+
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用于 Cu2 生物成像的比例光声纳米探针
DOI：
10.1021/acsami.8b20113
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发表时间：
2019
影响因子：
9.5
通讯作者：
Xiaoyuan Chen
中科院分区：
文献类型：
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作者：
Sheng Wang;Guochan Yu;Ying Ma;Zhen Yang;Yi Liu;Jing Wang;Xiaoyuan Chen
异常的铜含量涉及许多疾病，包括阿尔茨海默病和威尔逊病。传统的铜检测技术难以提供对铜的非侵入性和准确的深部组织检测。在这里，我们报告了光声（PA）纳米探针（NRh-IR-NMs）的比率PA成像的Cu 2+。纳米探针由作为指示剂的选择性Cu 2+响应探针（NRh）和作为内部参考的非响应染料（IR）组成。在Cu ~（2+）存在下，Cu ~（2+）选择性地诱导NRh的结构发生变化，导致716 nm处PA信号强度增量（Δ PA ~（716））增加。而归因于IR的Δ PA 834显示出可忽略的变化。因此，比率PA信号（Δ PA 716/Δ PA 834）可用作Cu 2+检测的指示剂。这种比率PA检测方法提供了一种具有高选择性和组织穿透深度的非侵入性技术，这是一种很有前途的工具，用于活生物体中Cu 2+的深层组织检测。
Aberrant copper contents implicate numerous diseases including Alzheimer’s disease and Wilson’s disease. Conventional copper detection technologies are difficult to offer non-invasive and accurate deep tissue detection of copper. Here, we report a photoacoustic (PA) nanoprobe (NRh-IR-NMs) for ratiometric PA imaging of Cu2+. The nanoprobe consists of a selective Cu2+-responsive probe (NRh) as the indicator and a nonresponsive dye (IR) as the internal reference. In the presence of Cu2+, a selective Cu2+-induced structure change of NRh would take place, resulting in the increase of PA signal intensity increment at 716 nm (ΔPA716). However, the ΔPA834 which attributes to IR shows negligible change. Therefore, the ratiometric PA signal (ΔPA716/ΔPA834) could be used as an indicator for Cu2+detection. This ratiometric PA detection method offers a noninvasive technology with high selectivity and tissue penetration depth, which is a promising tool for deep-tissue detection of Cu2+in living organisms.