Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
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DOI：
10.1007/978-1-4939-3148-4_14
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发表时间：
2016
影响因子：
--
通讯作者：
K. Valencia;F. Lecanda
中科院分区：
文献类型：
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作者：
K. Valencia;F. Lecanda
微泡和外来体被释放到细胞外环境中，并且在体液中可检测到。它们充当将货物转移到其他细胞/组织的独特载体。它们包含一组膜受体、细胞内蛋白质和核酸。在这里，我们描述了从细胞培养基或体液中分离和表征它们的基本技术。我们还描述了表征其货物（miRNA）及其蛋白质含量的关键技术。最后，我们提出了标记方法，它们在体外程序中使用，并在体内交付和随后的分析，其货物转移到多种细胞类型。
Microvesicles and exosomes are released to the extracellular milieu and are detectable in body fluids. They act as unique vehicles for cargo transfer to other cells/tissues. They contain a set of membrane receptors, intracellular proteins and nucleic acids. Here, we describe basic techniques for their isolation and characterization from cell culture media or body fluids. We also describe critical techniques for characterizing their cargo (miRNAs) and their protein content. Finally, we present labeling methods for their use in in vitro procedures and for their in vivo delivery and subsequent analysis of their cargo transfer to multiple cell types.