FOXL2 Is Regulated During the Bovine Estrous Cycle and Its Expression in the Endometrium Is Independent of Conceptus-Derived Interferon Tau
FOXL2 Is Regulated During the Bovine Estrous Cycle and Its Expression in the Endometrium Is Independent of Conceptus-Derived Interferon Tau
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DOI：
10.1095/biolreprod.112.101584
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发表时间：
2012-08-01
影响因子：
3.6
通讯作者：
Sandra, Olivier
中科院分区：
文献类型：
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作者：
Eozenou, Caroline;Carvalho, Anais Vitorino;Sandra, Olivier
FOXL2是一种翼螺旋结构域转录因子，是参与卵巢分化和生物学功能的关键基因。在最近的转录组学分析中，我们发现FOXL2在牛环形子宫内膜中的表达与环形子宫内膜中的表达不同。为了进一步了解FOXL2在该组织中的作用，我们测定了该转录因子在奶牛发情周期和妊娠建立期间的表达。子宫内膜FOXL2的表达在母体对妊娠的识别过程中没有变化（第16-20天）。通过牛体内模型和原代细胞培养，我们发现FOXL2不是干扰素-tau靶基因。FOXL2转录本和FOXL2蛋白在发情周期的第5天至第20天均有表达，在黄体溶解期表达水平显著升高。在犊牛中补充2天黄体酮可导致FOXL2蛋白水平明显下调，提示黄体酮对FOXL2表达有负面影响。免疫组化数据显示FOXL2定位于子宫内膜间质细胞和腺体细胞。在黄体溶解期收集的子宫内膜样本中，FOXL2亚细胞分布呈核状，而在黄体期和着床期的细胞核中未检测到FOXL2。总的来说，我们的发现提供了FOXL2参与子宫内膜组织生理调节的第一个证据。
FOXL2, a winged-helix/forkhead domain transcription factor, is a key gene involved in the differentiation and biological functions of the ovary. In a recent transcriptomic analysis, we found that FOXL2 expression in bovine caruncular endometrium was different from that in intercaruncular endometrium. In order to gain new insights into FOXL2 in this tissue, we determined the expression of this transcription factor during the estrous cycle and the establishment of pregnancy in cattle. The endometrial expression of FOXL2 did not vary during maternal recognition of pregnancy (Days 16-20). Using an in vivo bovine model and primary cell cultures, we showed that FOXL2 was not an interferon-tau target gene. Both FOXL2 transcript and protein were expressed from Day 5 to Day 20 of the estrous cycle, and their levels showed a significant increase during the luteolytic phase. A 2-day progesterone supplementation in heifers led to a clear down-regulation of FOXL2 protein levels, suggesting the negative impact of progesterone on FOXL2 expression. Immunohistochemistry data revealed the localization of FOXL2 in endometrial stromal and glandular cells. FOXL2 subcellular distribution was shown to be nuclear in endometrial samples collected during the luteolytic period, while it was not detected in nuclei during the luteal phase and at implantation. Collectively, our findings provide the first evidence that FOXL2 is involved in the regulation of endometrial tissue physiology.