Highly magnetizable superparamagnetic iron oxide nanoparticles embedded mesoporous silica spheres and their application for efficient recovery of DNA from agarose gel
Highly magnetizable superparamagnetic iron oxide nanoparticles embedded mesoporous silica spheres and their application for efficient recovery of DNA from agarose gel
复制标题
DOI：
10.1039/b812367e
复制
发表时间：
2009-01-01
影响因子：
--
通讯作者：
Yang, Wensheng
中科院分区：
文献类型：
--
作者：
Teng, Zhaogang;Li, Jun;Yang, Wensheng
采用溶胶-凝胶/乳液法制备了嵌有超顺磁性氧化铁纳米粒子的介孔二氧化硅微球。在0.4T的磁场下，所得超顺磁性二氧化硅球的饱和磁化强度为30 emu/g，在10 s内可被完全富集。透射电子显微镜（TEM）和扫描电子显微镜（SEM）成像表明，核壳结构的球。鲑鱼精子DNA的吸附和洗脱实验表明，超顺磁性介孔二氧化硅球具有良好的吸附DNA的能力。电泳实验表明，超顺磁性介孔二氧化硅球是合格的DNA片段从琼脂糖凝胶的有效回收。
Mesoporous silica spheres embedded with the superparamagnetic iron oxide nanoparticles were prepared by a new sol-gel/emulsion approach. The resulting superparamagnetic silica spheres with a saturation magnetization value of 30 emu/g could be enriched completely within 10 s under the application of a 0.4 T magnet. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) imaging indicated a core-shell structure of the spheres. Adsorption and elution experiments of salmon sperm DNA showed an excellent ability of the superparamagnetic mesoporous silica spheres to adsorb DNA. Electrophoresis experiments show that the superparamagnetic mesoporous silica spheres are qualified for efficient recovery of DNA fragments from agarose gel.