SIMILARITIES IN TRANSTHYRETIN GENE-EXPRESSION AND DIFFERENCES IN TRANSCRIPTION FACTORS - LIVER AND YOLK-SAC COMPARED TO CHOROID-PLEXUS
SIMILARITIES IN TRANSTHYRETIN GENE-EXPRESSION AND DIFFERENCES IN TRANSCRIPTION FACTORS - LIVER AND YOLK-SAC COMPARED TO CHOROID-PLEXUS
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DOI：
10.1073/pnas.87.17.6589
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发表时间：
1990-09-01
影响因子：
11.1
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
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作者：
COSTA, RH;VANDYKE, TA;DARNELL, JE
血清甲状腺素结合蛋白，甲状腺素运载蛋白（TTR），是由肝细胞和脉络丛上皮细胞在成人和卵黄囊细胞在胚胎发育。四种肝细胞核因子（HNF-1、-3、-4和C/EBP）存在于肝脏中，但不存在于大多数其他成人组织中，它们与TTR基因中足以指导转基因表达的DNA位点结合。这些蛋白质中的三种也在卵黄囊细胞中发现，卵黄囊细胞也表达转基因构建体，其在脉络丛中不活跃，并且产生TTR的脉络丛肿瘤缺乏三种肝脏富集的DNA结合蛋白。我们的结论是，TTR的细胞特异性表达的调节至少部分由差异细胞分布的正作用转录因子。
The serum thyroxine-binding protein, transthyretin (TTR), is made by hepatocytes and by choroid plexus epithelium in adults and by yolk sac cells in embryogenesis. Four hepatocyte nuclear factors (HNF-1, -3, and -4 and C/EBP) that are present in liver but not in most other adult tissues bind DNA sites in the TTR gene that are sufficient to direct transgenic expression. Three of these proteins were also found in yolk sac cells, which also express the transgenic construct is not active in the choroid plexus and a TTR-producing choroid plexus tumor lacks three of the liver-enriched DNA-binding proteins. We conclude that cell-specific expression of TTR is regulated at least in part by the differential cellular distribution of positive-acting transcription factors.