CHANGES IN INTESTINAL-ABSORPTION OF NUTRIENTS AND BRUSH-BORDER GLYCOPROTEINS AFTER TOTAL PARENTERAL-NUTRITION IN RATS
CHANGES IN INTESTINAL-ABSORPTION OF NUTRIENTS AND BRUSH-BORDER GLYCOPROTEINS AFTER TOTAL PARENTERAL-NUTRITION IN RATS
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DOI：
10.1136/gut.33.4.484
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发表时间：
1992-04-01
期刊：
影响因子：
24.5
通讯作者：
TSUCHIYA, M
中科院分区：
文献类型：
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作者：
MIURA, S;TANAKA, S;TSUCHIYA, M
本实验观察了全胃肠外营养对大鼠小肠营养吸收和刷状缘膜糖蛋白的影响。在全胃肠外营养大鼠中，观察到刷状缘酶的活性显著降低，主要在肠的近端和中段。空肠段半乳糖灌流显示，全肠外营养治疗显著抑制了己糖的吸收，而对甘氨酸或二肽、甘氨酰甘氨酸的肠吸收无明显影响。当通过[H-3]-葡萄糖胺或[H-3]-岩藻糖掺入空肠袢检查刷状缘膜糖蛋白谱时，观察到全胃肠外营养治疗后刷状缘膜糖蛋白谱发生显著变化，特别是在超过120 kDa的高分子量范围内，表明糖蛋白合成对管腔物质的强烈依赖性。特异性抗体检测刷状缘膜蔗糖酶异麦芽糖酶分子量，全肠外营养组与对照组无显著性差异。此外，选择性光亲和标记法检测到的小肠刷状缘膜特异性钠-葡萄糖协同转运蛋白的分子量在全胃肠外营养大鼠中没有改变。这可能是因为长期不进食可引起刷状缘膜糖蛋白和小肠吸收能力的显著生化变化，但在所有刷状缘膜糖蛋白中未观察到这些变化。
The effect of total parenteral nutrition on nutrients absorption and glycoprotein changes of brush border membrane was examined in rat small intestine. In total parenteral nutrition rats, a marked decrease in activity of brush border enzymes was observed mainly in the proximal and middle segments of the intestine. Galactose perfusion of jejunal segment showed that hexose absorption was significantly inhibited, while intestinal absorption of glycine or dipeptide, glycylglycine was not significantly affected by total parenteral nutrition treatment. When brush border-membrane glycoprotein profile was examined by [H-3]-glucosamine or [H-3]-fucose incorporation into jejunal loops, significant changes were observed in the glycoprotein pattern of brush border membrane especially in the high molecular weight range over 120 kDa after total parenteral nutrition treatment, suggesting strong dependency of glycoprotein synthesis on luminal substances. Molecular weight of sucrase isomaltase in brush border membrane detected by specific antibody showed no significant difference, however, in total parenteral nutrition and control rats. Also, molecular weight of specific sodium glucose cotransporter of intestinal brush border membrane detected by selective photoaffinity labelling was not altered in total parenteral nutrition rats. It may be that prolonged absence of oral food intake may produce significant biochemical changes in brush border membrane glycoprotein and absorptive capacity of small intestine, but these changes were not observed in all brush border membrane glycoproteins.