Uterine and Placental Angiogenesis in the Australian Skinks, Ctenotus taeniolatus, and Saiphos equalis
Uterine and Placental Angiogenesis in the Australian Skinks, Ctenotus taeniolatus, and Saiphos equalis
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DOI：
10.1002/ar.21052
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发表时间：
2010-05-01
影响因子：
2
通讯作者：
Thompson, Michael B.
中科院分区：
文献类型：
--
作者：
Parker, Scott L.;Manconi, Frank;Thompson, Michael B.
胎生的进化需要改变多种综合生理特征，以支持怀孕期间的胚胎发育。胚胎在怀孕期间的生长是依赖于子宫血管系统的能力，以满足不断增加的胚胎在整个妊娠期的氧气需求。我们测试了一个假设，即子宫血管的总表面积随着胚胎的生长而增加，因此它的氧气需求，在妊娠期间。我们使用免疫荧光和激光扫描共聚焦显微镜，定量研究了卵生小毛犀（Ctenotus taeniolatus）和卵生小毛犀（Saiphos equalis）在妊娠期间的子宫微血管密度和形态。在胚胎半球子宫血管密度（Nv）和血管长度密度（Lv）上，带卷卷绦虫的血管密度（Nv）和长度密度（Lv）分别比等叶绦虫低23%和17%，血管表面积不随胚胎阶段的变化而变化。对于平叶菊，总的Nv、Lv和血管直径（Dv）在妊娠前半期没有变化，但在妊娠后期的近期胚胎阶段增加了36% （Nv）、44% （Lv）和60% （Dv）。胚期不同，毛囊尿囊膜的绝对尺寸增大，但血管密度无显著差异。妊娠后期子宫血管密度的显著增加与胚胎质量的快速增长和代谢率的增加相一致。子宫血管床的扩张与胚胎的大小和新陈代谢相一致，可能是胎生进化的重要过渡步骤。中国生物医学工程学报，2009,29(3):829-838。(C) 2010 Wiley-Liss, Inc。
The evolution of viviparity requires modifications to multiple integrated physiological features to support embryonic development during pregnancy. Embryonic growth during pregnancy is dependent upon the capacity of the uterine vascular system to satisfy increasing embryonic oxygen demand throughout gestation. We tested the hypothesis that total surface area of uterine blood vessels increases in concert with embryonic growth, and hence its oxygen demand, during gestation. We used immunofluorescence and laser-scanning confocal microscopy to quantify uterine microvascular density and morphology during gestation in the oviparous skink Ctenotus taeniolatus and in Saiphos equalis, a skink species with prolonged egg retention. For C. taeniolatus, vessel density (Nv) and vessel length-density (Lv) in the embryonic hemisphere of the uterus is 23% and 17% less, respectively, than that of S. equalis and vascular surface-area does not differ as a function of embryo stage. For S. equalis, overall Nv, Lv, and vessel diameter (Dv), does not change during the first half of gestation but increases by 36% (Nv), 44% (Lv), and 60% (Dv) by near-term embryo stages late in gestation. The chorioallantoic membrane of S. equalis increases in absolute size but vascular density does not differ as a function of embryo stage. The marked increase in uterine vascular density during late gestation coincides with the phase of rapid growth in embryo mass and concomitant increase in metabolic rate. Expansion of the uterine vascular bed in concert with embryo size and metabolism is likely to be an important transitional step in the evolution of viviparity. Anat Rec, 293:829-838, 2010. (C) 2010 Wiley-Liss, Inc.