A computerized grid walking system for evaluating the accuracy of locomotion in rats.
A computerized grid walking system for evaluating the accuracy of locomotion in rats.
复制标题
用于评估大鼠运动准确性的计算机网格行走系统。
DOI：
10.1016/s0165-0270(05)80003-2
复制
发表时间：
1993
影响因子：
3
通讯作者：
Holets,VR
中科院分区：
文献类型：
--
作者：
Prakriya,M;McCabe,PM;Holets,VR
开发并评估了基于微计算机的系统，该系统采用光电装置来记录网格行走测试范例中的大鼠运动和脚步。从系统获得的行为数据包括：网格上走过的距离、走过该距离所需的时间、脚步次数、脚步次数、脚步位置、脚步持续时间以及脚步是由后肢还是前肢造成的。通过将从录像带分析获得的数据与从计算机化系统获得的数据进行比较来进行系统的验证。通过这两种方法获得的数据之间的相关系数，一位观察者为 0.92，另一位观察者为 0.84，两位观察者平均值为 0.88。还进行了一项实验研究，其中对一组大鼠进行了脊髓背侧半切损伤的治疗。病变组的动物穿过跑道的时间与对照组动物相同，但每次穿过跑道的脚步量更多，并且每次脚步的持续时间更长。这些研究验证了计算机系统有效检测精细运动缺陷的能力，表明它是评估实验大鼠神经功能障碍的可行工具。
A microcomputer-based system employing photoelectric devices to record rat movements and footfalls in the grid walking test paradigm was developed and evaluated. Behavioral data obtained from the system were: distance traversed on the grid, time taken to traverse the distance, number of footfalls, times of footfalls, positions of footfalls, durations of footfalls, and whether the footfall was due to a hind or a fore limb. Validation of the system was performed by comparing the data obtained from the videotape analysis with that obtained from the computerized system. Correlation coefficients between the data obtained from the two methods were found to be 0.92 for one observer, 0.84 for a second observer, and 0.88 with the mean of the two observers. An experimental study in which a group of rats was administered dorsal hemisection lesions of the spinal cord was also conducted. Animals in the lesion group took the same amount of time to cross the runway as the control animals, but made more footfalls per crossing and had longer durations per footfall. The studies validate the capacity of the computerized system to efficiently detect fine locomotory deficits, suggesting that it is a viable tool for the evaluation of neurological dysfunctions in experimental rats.