MITOCHONDRIAL-FUNCTION IN THE PRESENCE OF MYOGLOBIN
MITOCHONDRIAL-FUNCTION IN THE PRESENCE OF MYOGLOBIN
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DOI：
10.1152/jappl.1982.53.5.1116
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
WITTENBERG, BA
中科院分区：
文献类型：
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作者：
COLE, RP;SUKANEK, PC;WITTENBERG, BA
在稳态供氧条件下，研究了肌红蛋白对离体大鼠骨骼肌线粒体耗氧和ATP生成的影响。提出了一种方法，用于确定在O2结合蛋白的存在下的稳态O2消耗。线粒体悬浮液中消耗的O2通过从流动的气相转移而不断补充。用O2电极测量液相O2压力;气相O2浓度保持恒定在一系列固定值。从系统的特征响应时间和稳态气相和液相O2浓度的差异确定O2消耗。在己糖激酶的存在下，从G-6-P的产生确定ATP的产生。在稳态线粒体O2消耗过程中，当肌红蛋白存在时，液相中的O2压力增加。功能肌红蛋白存在于溶液中没有影响线粒体呼吸和ATP生产的关系，液相O2压力。肌红蛋白在该系统中的功能是增强O2进入含肌红蛋白相的通量。肌红蛋白可能以类似的方式在肌肉中通过增加进入肌细胞的O2通量发挥作用。
The effect of myoglobin on O2 consumption and ATP production by isolated rat skeletal muscle mitochondria was studied under steady-state conditions of O2 supply. A method is presented for the determination of steady-state O2 consumption in the presence of O2-binding proteins. O2 consumed in suspensions of mitochondria was replenished continuously by transfer from a flowing gas phase. Liquid-phase O2 pressure was measured with an O2 electrode; the gas-phase O2 concentration was held constant at a series of fixed values. O2 consumption was determined from the characteristic response time of the system and the difference in the steady-state gas- and liquid-phase O2 concentrations. ATP production was determined from the generation of G-6-P in the presence of hexokinase. During steady-state mitochondrial O2 consumption, the O2 pressure in the liquid phase in enhanced when myoglobin in present. Functional myoglobin present in the solution had no effect on the relation of mitochondrial respiration and ATP production to liquid-phase O2 pressure. Myoglobin functions in this system to enhance the flux of O2 into the myoglobin-containing phase. Myoglobin may function in a similar fashion in muscle by increasing O2 flux into myocytes.