Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element
Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element
复制标题
莱姆病螺旋体的圆形和线性质粒具有广泛的同源性：重复DNA元件的表征
DOI：
10.1128/jb.178.8.2287-2298.1996
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发表时间：
1996
影响因子：
3.2
通讯作者：
J. Meyer
中科院分区：
文献类型：
--
作者：
W. Zückert;J. Meyer
We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A and ORF-B. Five additional ORFs can be found on the slightly less homologous flanking sequences: ORF-G on the opposite strand upstream of ORF-A, and ORF-C, ORF-D, ORF-E, and ORF-F downstream of ORF-B. The 4.6-kb-long element containing ORF-A through ORF-E is flanked by approximately 180-bp-long imperfect inverted repeats (IRs). The putative gene product of ORF-C displays homology to proteins involved in plasmid maintenance in a number of gram-positive and gram-negative bacteria. ORF-E features several short, highly homologous direct repeats. ORF-A, ORF-B, and ORF-D are homologous to three ORFs on a recently described 8.3-kb circular plasmid of Borrelia afzelii Ip21 that are flanked by similar IRs (J. J. Dunn, S. R. Buchstein, L.-L. Butler, S. Fisenne, D. S. Polin, B. N. Lade, and B. J. Luft, J. Bacteriol. 176:2706-2717,1994). ORF-C and ORF-E, however, are missing from this region on the Ip21 plasmid. Furthermore, the repeated DNA element as defined by the IRs is present in opposite orientations relative to the flanking sequences on the B31 and Ip21 plasmids.
回归热病原赫氏疏螺旋体具有多个染色体拷贝和线性质粒。
影响因子：
3.3
作者：
Kitten,T;Barbour,AG
通讯作者：
Barbour,AG
影响因子：
2.9
作者：
CHOMCZYNSKI, P
通讯作者：
CHOMCZYNSKI, P