Intake of Red Wine Increases the Number and Functional Capacity of Circulating Endothelial Progenitor Cells by Enhancing Nitric Oxide Bioavailability
Intake of Red Wine Increases the Number and Functional Capacity of Circulating Endothelial Progenitor Cells by Enhancing Nitric Oxide Bioavailability
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DOI：
10.1161/atvbaha.109.200618
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发表时间：
2010-04-01
影响因子：
8.7
通讯作者：
Lin, Shing-Jong
中科院分区：
文献类型：
--
作者：
Huang, Po-Hsun;Chen, Yung-Hsiang;Lin, Shing-Jong
目的：饮用红酒（RW）与心血管事件的减少有关，但有关潜在调节机制的数据有限。本研究测试了摄入 RW 可能通过增强一氧化氮生物利用度来促进循环内皮祖细胞 (EPC) 水平和功能的假设。方法和结果 - 80 名健康的年轻受试者被随机分配，每天饮用水 (100 mL)、RW (100 mL)、啤酒 (250 mL) 或伏特加 (30 mL)，持续 3 周。流式细胞术用于量化循环 EPC 数量，体外测定用于评估 EPC 功能。摄入RW后，由血流介导的血管舒张测定的内皮功能显着增强；然而，摄入水、啤酒或伏特加后，它保持不变。仅在RW组中，循环EPC（定义为KDR（+）CD133（+），CD34（+）CD133（+），CD34（+）KDR（+））和EPC集落形成单位的数量显着增加（所有P
Objective-Red wine (RW) consumption has been associated with a reduction of cardiovascular events, but limited data are available on potential mediating mechanisms. This study tested the hypothesis that intake of RW may promote the circulating endothelial progenitor cell (EPC) level and function through enhancement of nitric oxide bioavailability.Methods and Results-Eighty healthy, young subjects were randomized and assigned to consume water (100 mL), RW (100 mL), beer (250 mL), or vodka (30 mL) daily for 3 weeks. Flow cytometry was used to quantify circulating EPC numbers, and in vitro assays were used to evaluate EPC functions. After RW ingestion, endothelial function determined by flow-mediated vasodilation was significantly enhanced; however, it remained unchanged after water, beer, or vodka intake. There were significantly increased numbers of circulating EPC (defined as KDR(+)CD133(+), CD34(+)CD133(+), CD34(+)KDR(+)) and EPC colony-forming units only in the RW group (all P