Oxygen transport and consumption by suspended cells in microgravity: A multiphase analysis
Oxygen transport and consumption by suspended cells in microgravity: A multiphase analysis
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DOI：
10.1002/bit.21542
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发表时间：
2008-01-01
影响因子：
3.8
通讯作者：
Banerjee, Rupak K.
中科院分区：
文献类型：
--
作者：
Kwon, Ohwon;Devarakonda, Surendra B.;Banerjee, Rupak K.
应操作用于细胞/组织培养的旋转生物反应器，以获得足够的营养转移并避免对培养材料的损坏。因此，本研究的目的是确定微珠/细胞分布的适当悬浮条件，并评价旋转壁容器（RWV）生物反应器中的氧转运。采用欧拉-欧拉多相流方程和氧输运方程对RWV生物反应器进行了数值分析。珠粒尺寸和旋转速度是计算中的控制变量。目前的结果表明，随着珠粒/细胞尺寸的增加，适当悬浮液的转速需要增加：200 μ m为10 rpm; 300 μ m为12 rpm; 400 μ m为14 rpm; 600 μ m为18 rpm。当转速和珠粒尺寸从10 rpm/200 μ m增加到18 rpm/600 μ m时，由于与10 rpm/200 μ m的情况相比，18 rpm/600 μ m的情况下的高对流流动，容器的80%中间区中的平均氧浓度在1小时旋转后增加了约85%。本研究结果可作为RWV生物反应器的操作参数设置的标准，如珠的大小和转速，根据细胞聚集体的生长。此外，它可能会提供一个先进的悬浮生物反应器的设计参数的三维工程细胞和组织培养。
A rotating bioreactor for the cell/tissue culture should be operated to obtain sufficient nutrient transfer and avoid damage to the culture materials. Thus, the objective of the present study is to determine the appropriate suspension conditions for the bead/cell distribution and evaluate oxygen transport in the rotating wall vessel (RWV) bioreactor. A numerical analysis of the RWV bioreactor is conducted by incorporating the Eulerian-Eulerian multiphase and oxygen transport equations. The bead size and rotating speed are the control variables in the calculations. The present results show that the rotating speed for appropriate Suspensions needs to be increased as the size of the bead/cell increases: 10 rpm for 200 mu m; 12 rpm for 300 mu m; 14 rpm for 400 mu m; 18 rpm for 600 mu m. As the rotating speed and the bead size increase from 10 rpm/200 mu m to 18 rpm/600 mu m, the mean oxygen concentration in the 80% midzone of the vessel is increased by similar to 85% after 1-h rotation due to the high convective flow for 18 rpm/600 mu m case as compared to 10 rpm/200 pm case. The present results may serve as criteria to set the operating parameters for a RWV bioreactor, such as the size of beads and the rotating speed, according to the growth of cell aggregates. In addition, it might provide a design parameter for an advanced suspension bioreactor for 3-D engineered cell and tissue cultures.