2-STAGE SKIN CARCINOGENESIS BY SYSTEMIC INITIATION OF PREGNANT MICE WITH 7,12-DIMETHYLBENZ(A)ANTHRACENE DURING GESTATION DAYS 6-20 AND POSTNATAL PROMOTION OF THE F1-GENERATION WITH THE PHORBOL ESTER 12-TETRADECANOYLPHORBOL-13-ACETATE
2-STAGE SKIN CARCINOGENESIS BY SYSTEMIC INITIATION OF PREGNANT MICE WITH 7,12-DIMETHYLBENZ(A)ANTHRACENE DURING GESTATION DAYS 6-20 AND POSTNATAL PROMOTION OF THE F1-GENERATION WITH THE PHORBOL ESTER 12-TETRADECANOYLPHORBOL-13-ACETATE
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DOI：
10.1007/bf00410789
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发表时间：
1980-01-01
影响因子：
3.6
通讯作者：
HESSE, B
中科院分区：
文献类型：
--
作者：
GOERTTLER, K;LOEHRKE, H;HESSE, B
对DMBA[7，12-二甲基苯并（a）蒽]-TPA[12-十四酰基佛波醇-13-乙酸酯]介导的2阶段皮肤致癌作用实验进行了修改，其中在妊娠期间用致癌物对妊娠小鼠进行一次全身性处理。胃内给药时间为胎仔第6、8和10-20天。妊娠小鼠的对照组接受重复剂量（从第8-20天）的芝麻油。在12周龄时，将后代分为2组，其中一组完全不治疗，而另一组接受TPA应用超过26周。观察TPA促进后的皮肤肿瘤发展。肿瘤率和肿瘤产量增加，潜伏期逐渐减少，在促进亚组与推迟启动到以后的胎儿期。产前发育的第19天是经胎盘启动的最敏感时期;在第20天启动导致肿瘤率和产量显著降低。引发皮肤肿瘤的能力和引发的程度可以与表皮的器官发生及其在子宫内的增殖速率相关。
The DMBA[7,12-dimethylbenz(a)anthracene]-TPA[12-tetradecanoylphorbol-13-acetate]-mediated 2-stage skin carcinogenesis experiment was modified in that pregnant mice were systemically treated once during pregnancy with the carcinogen. Intragastric application times were fetal days 6, 8 and 10-20. A control group of pregnant mice received repeated doses (from days 8-20) of sesame oil. At the age of 12 wk, the offspring were divided into 2 groups, one of which was left completely untreated while the other received TPA applications over 26 wk. Skin tumor development after TPA promotion was observed. Tumor rates and tumor yields increased and latency periods decreased progressively in the promoted subgroups with the postponement of initiation to later fetal periods. Day 19 of prenatal development was the most sensitive period to transplacental initiation; initiation at day 20 led to a significant decrease in tumor rate and yield. The capability to initiate skin tumors and the extent of initiation can be correlated to the organogenesis of the epidermis and its proliferative rate in utero.