An evidential example of airborne bacteria in a crowded, underground public concourse in Tokyo
An evidential example of airborne bacteria in a crowded, underground public concourse in Tokyo
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DOI：
10.1016/j.atmosenv.2004.09.030
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发表时间：
2005
影响因子：
5
通讯作者：
Kaoruko Seino;T. Takano;Keiko Nakamura;Masafumi Watanabe
中科院分区：
文献类型：
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作者：
Kaoruko Seino;T. Takano;Keiko Nakamura;Masafumi Watanabe
我们研究了空气中的细菌在东京的地下大厅，并调查了影响细菌计数的条件。空气中的细菌收集使用撞击采样器。对平板计数琼脂（PCA）和含5%羊血的哥伦比亚粘菌素-萘啶酸琼脂（CNA琼脂）上的菌落进行计数。PCA和CNA琼脂上细菌计数（CFU m − 3）的范围、几何平均值和95% CI分别为150 - 1380、456、382 - 550和50 - 990、237、182 - 309。PCA上的细菌计数与行人数量（r = 0.89）、相对湿度（r = 0.70）和空气中的灰尘（PM5.0）（r = 0.73）显著相关。多元回归分析结果表明，在排除相对湿度和空气尘埃的影响后，行人数量与PCA上的细菌数量之间存在独立的正相关关系（p <0.01）。对CNA琼脂上的细菌计数进行统计分析，获得了类似的结果。PCA和CNA琼脂上革兰阳性球菌占优势。表皮葡萄球菌和微球菌属。在本研究鉴定的11属19种中，以小孢子虫属的种类最多。考虑到所鉴定的物种的模式以及行人数量与细菌计数之间的显著独立关联，拥挤的地下大厅中的空气细菌主要来源于在地下大厅中行走的行人。这项研究给出了东京一个拥挤的地下公共空间空气中细菌状况的证据。
We examined airborne bacteria in an underground concourse in Tokyo and investigated conditions that influenced bacterial counts. Airborne bacteria were collected by using an impactor sampler. Colonies on plate count agar (PCA) and Columbia colistin–nalidixic acid agar with 5% sheep blood (CNA agar) were enumerated. The range, geometric mean, and 95% CI of the bacterial counts (CFU m−3) on PCA and CNA agar were 150–1380, 456, 382–550 and 50–990, 237, 182–309, respectively. Bacterial counts on PCA significantly correlated with number of the pedestrians (r=0.89), relative humidity (r=0.70) and airborne dust (PM5.0) (r=0.73). Results of a multiple regression indicated independent positive association between the number of pedestrians and bacterial counts on PCA (p<0.01) after excluding the influence of relative humidity and airborne dust. Similar results were obtained with the statistical analysis for the counts of bacteria on CNA agar. Gram-positive cocci were dominant on PCA and CNA agar. Staphylococcus epidermidis and Micrococcus spp. were dominant among the 11 genera and 19 species identified in the present study. Considering the pattern of identified species and the significant independent association between number of pedestrians and bacterial counts, airborne bacteria in a crowded underground concourse were mostly originated from the pedestrians who were walking in the underground concourse. This study gave an evidential example of bacterial conditions in the air of an underground crowded public space in Tokyo.