Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression
Consequences of conceptus exposure to colony-stimulating factor 2 on survival, elongation, interferon-τ secretion, and gene expression
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DOI：
10.1530/rep-10-0511
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发表时间：
2011-05-01
期刊：
影响因子：
3.8
通讯作者：
Hansen, Peter J.
中科院分区：
文献类型：
--
作者：
Loureiro, Barbara;Block, Jeremy;Hansen, Peter J.
暴露牛的概念，以集落刺激因子2（CSF 2）从第5天到第7天的发展可以增加的百分比，发展到术语的转让概念。本实验的目的是了解CSF 2增加胚胎和胎儿存活的机制。从授精后第5天至第7天，在存在或不存在10 ng/ml CSF 2的情况下体外产生受孕，转移到奶牛体内，并在妊娠第15天从子宫冲洗。有一种趋势（P=0.07），即接受CSF 2给药孕体的奶牛中孕体恢复的奶牛比例更高（对照组为35%，CSF 2组为66%）。子宫冲洗液中的抗病毒活性（衡量孕体分泌的干扰素-tau（IFNT 2）量）往往大于接受CSF 2处理孕体的奶牛。当仅考虑具有可检测到的抗病毒活性的奶牛时，这种差异接近显著性（P=0.07）。此外，CSF 2还增加了IFN-γ 2（P=0.08）和角蛋白18（P
Exposure of bovine conceptuses to colony-stimulating factor 2 (CSF2) from days 5 to 7 of development can increase the percentage of transferred conceptuses that develop to term. The purpose of this experiment was to understand the mechanism by which CSF2 increases embryonic and fetal survival. Conceptuses were produced in vitro in the presence or absence of 10 ng/ml CSF2 from days 5 to 7 after insemination, transferred into cows, and flushed from the uterus at day 15 of pregnancy. There was a tendency (P=0.07) for the proportion of cows with a recovered conceptus to be greater for those receiving a CSF2-treated conceptus (35% for control versus 66% for CSF2). Antiviral activity in uterine flushings, a measure of the amount of interferon-tau (IFNT2) secreted by the conceptus, tended to be greater for cows receiving CSF2-treated conceptuses than for cows receiving control conceptuses. This difference approached significance when only cows with detectable antiviral activity were considered (P=0.07). In addition, CSF2 increased mRNA for IFNT2 (P=0.08) and keratin 18 (P