Effect of Brimonidine on Retinal Ganglion Cell Survival in an Optic Nerve Crush Model
Effect of Brimonidine on Retinal Ganglion Cell Survival in an Optic Nerve Crush Model
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DOI：
10.1016/j.ajo.2008.08.005
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发表时间：
2009-02-01
影响因子：
4.2
通讯作者：
Jonas, Jost B.
中科院分区：
文献类型：
--
作者：
Ma, Ke;Xu, Liang;Jonas, Jost B.
目的：探讨溴莫尼定对视神经挤压模型视网膜神经节细胞存活的影响。设计：实验动物研究。方法：将24只Sprague-Dawley大鼠分为研究组（8只）和对照组（12只），分别腹腔注射溴莫尼定（1 mg/kg）和生理盐水。所有注射均在视神经崩溃前1小时进行，之后每天进行。每只动物的右视神经被一个功率为40克的微型夹子压碎60秒。视神经挤压后23天，在脑上丘两侧注射3%氟金逆行标记视网膜神经节细胞。视神经损伤4周后，这些动物被处死。从视网膜平面支架上拍摄的照片被评估视网膜神经节细胞的数量和密度。结果：视神经病变右眼视网膜神经节细胞密度，溴莫尼定研究组（1281 +/- 189细胞/mm(2)）高于对照组（1060 +/- 148细胞/mm(2)），差异有统计学意义（P = 0.02）。相应的，研究组的存活率（右眼视网膜神经节细胞密度与左眼细胞密度之比）高于对照组（61.0% +/- 6.0% vs 53.5 +/- 8.0%），差异有统计学意义（P = 0.027）。结论：实验性和标准化视神经压迫大鼠治疗前后每日预防性注射溴莫尼定可提高视网膜神经节细胞的存活率。[J]中华眼科杂志，2009;47:326-331。(C) 2009年Elsevier Inc.版权所有。)
PURPOSE: To investigate the effect of brimonidine on the retinal ganglion cell survival in an optic nerve crush model.DESIGN: Experimental animal study.METHODS: Twenty-four Sprague-Dawley rats were divided into a study group of eight animals receiving intraperitoneal injections of brimonidine (I mg/kg) and into a control group of 12 animals receiving intraperitoneal saline injections. All injections were performed one hour before the optic nerve crash and daily afterwards. For each animal, the right optic nerve was crushed for 60 seconds by a microclip with 40-g power. At 23 days after the optic nerve crush, the retinal ganglion cells were retrogradely labeled by injecting 3% fluorogold into both sides of the superior colliculus of the brain. At four weeks after the optic nerve crush, the animals were sacrificed. Photographs taken from retinal flat mounts were assessed for number and density of the retinal ganglion-cells.RESULTS: The retinal ganglion cell density of the right eyes with an optic nerve lesion was statistically significantly (P = .02) higher in the brimonidine study group (1281 +/- 189 cells/mm(2)) than in the control group (1060 +/- 148 cells/mm(2)). Correspondingly, the survival rate (ratio of retinal ganglion cell density in the right eye divided by cell density in the left eye) was statistically significantly (P = .027) higher in the study group than in the control group (61.0% +/- 6.0% vs 53.5 +/- 8.0%).CONCLUSION: Intraperitoneal injections of brimonidine given prophylactically prior to and posttreatment daily after an experimental and standardized optic nerve crush in rats were associated with a higher survival rate of retinal ganglion cells. (Am J Ophthalmol 2009;147:326-331. (C) 2009 by Elsevier Inc. All rights reserved.)