SUPPRESSION OF HUMAN-MELANOMA CELL-GROWTH AND METASTASIS BY THE MELANOMA-ASSOCIATED ANTIGEN CD63 (ME491)
SUPPRESSION OF HUMAN-MELANOMA CELL-GROWTH AND METASTASIS BY THE MELANOMA-ASSOCIATED ANTIGEN CD63 (ME491)
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DOI：
10.1002/ijc.2910620523
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发表时间：
1995-09-04
影响因子：
6.4
通讯作者：
HERSEY, P
中科院分区：
文献类型：
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作者：
RADFORD, KJ;MALLESCH, J;HERSEY, P
CD63已被许多不同的单克隆抗体（mab）在人类黑色素瘤细胞中鉴定出来。单克隆抗体的研究表明，在痣和早期形式的皮肤黑色素瘤中表达最明显，在垂直生长阶段和转移性病变中表达减少。为了进一步研究CD63在黑色素瘤进展中的作用，使用episomal载体将基因组CD63转染到CD63阴性的人黑色素瘤细胞系中。稳定转染的黑色素瘤细胞在体外具有与控制转染的黑色素瘤细胞相似的生长速率，但当皮下注射到胸腺裸鼠时，生长速率要低得多。经静脉注射的cdb3转染的细胞在腹腔和皮下部位转移的数量也减少。抗CD63的单克隆抗体不影响体外转染cdb3的黑色素瘤细胞的生长。我们的结果证实了先前使用h -ros转化的NIH3T3成纤维细胞的研究，并表明CD63可能在人类黑色素瘤中具有肿瘤抑制基因的作用，可以限制黑色素瘤的侵袭和进展。(C) 1995 Wiley-Liss, Inc。
CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the role of CD63 in progression of melanoma, genomic CD63 was transfected into a CD63 negative human melanoma cell line using an episomal vector. The stable transfected melanoma cells had similar growth rates to control transfected melanoma cells in vitro but much lower growth rates when injected intradermally into athymic nude mice. The CDb3-transfected cells also had a reduced number of metastases in the peritoneal cavity and subcutaneous sites when injected intravenously. MAb against CD63 did not influence the growth of CDb3-transfected melanoma cells in vitro. Our results confirm previous studies using H-ros-transformed NIH3T3 fibroblasts and suggest that CD63 may have a role as a tumor suppressor gene in human melanoma that acts to limit invasion and progression of melanoma. (C) 1995 Wiley-Liss, Inc.