NEGATIVE STAINING OF INTEGRAL MEMBRANE-PROTEINS
NEGATIVE STAINING OF INTEGRAL MEMBRANE-PROTEINS
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DOI：
10.1016/0739-6260(91)90054-4
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
BOEKEMA, EJ
中科院分区：
文献类型：
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作者：
BOEKEMA, EJ
这篇综述介绍了分离的完整膜蛋白的负染色方面。 去污剂在膜蛋白的分离中以及在它们在水溶液中的溶解度中起着核心作用。 膜蛋白和非离子去污剂的混合胶束的样品可以很容易地制备，只要去污剂浓度保持在临界胶束浓度以上。 以参与光合作用过程的膜蛋白为例，说明它们与不同去污剂的相互作用。 在负染色时，膜蛋白和洗涤剂的混合胶束显示出特征性的顶视图和侧视图投影。 在它们的侧面，混合胶束可以容易地聚集成线。
This review describes aspects of negative staining of isolated integral membrane proteins. Detergents play a central role in the isolation of membrane proteins and also in their solubility in aqueous solutions. Specimens of mixed micelles of membrane proteins and non-ionic detergents can be easily prepared as long as the detergent concentration remains above the critical micellar concentration. Membrane proteins involved in the process of photosynthesis have been taken as examples to illustrate their interaction with different detergents. Upon negative staining, mixed micelles of membrane proteins and detergents show characteristic top and side view projections. On their sides, mixed micelles can easily aggregate into strings.