Cellular encapsulation and melanization are enhanced by immulectins, pattern recognition receptors from the tobacco hornworm Manduca sexta
Cellular encapsulation and melanization are enhanced by immulectins, pattern recognition receptors from the tobacco hornworm Manduca sexta
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DOI：
10.1016/j.dci.2005.05.005
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Yu, XQ
中科院分区：
文献类型：
--
作者：
Ling, EJ;Yu, XQ
昆虫对寄生虫的有效先天免疫反应是包被和黑化。在细胞包封过程中，囊体的形成需要浆细胞和粒细胞，这是鳞翅目昆虫中最丰富的两种血细胞。我们开发了一种体外包封实验来阐明免疫素在包封中的功能。免疫素是c型（钙依赖性）凝集素超家族的成员，它们在烟草角虫的先天免疫中作为体液模式识别受体起作用。我们证明免疫素包被琼脂糖珠增强了体外细胞包封。免疫组化素-1增强包封，但不增强黑化。当琼脂糖珠被免疫球蛋白包被时，缺乏浆细胞的分离血细胞发生包被。然而，包膜珠的聚集需要浆细胞。我们还发现丝氨酸蛋白酶抑制剂如对氨基苯基甲基磺酰氟（p-APMSF）抑制免疫凝集素包被琼脂糖珠的细胞包封。我们的研究结果表明，体液模式识别受体可能会增强细胞的包封和黑色素化。(c) 2005 Elsevier Ltd版权所有。
An effective innate immune response against parasites in insects is encapsulation followed by melanization. In cellular encapsulation, formation of capsules requires plasmatocytes and granulocytes, the two most abundant hemocytes in lepidopteran insects. We have developed an in vitro encapsulation assay to elucidate functions of immulectins in encapsulation. Immulectins are members of the C-type (calcium-dependent) lectin superfamily, and they function as humoral pattern recognition receptors in innate immunity of the tobacco hornworm Manduca sexta. We demonstrate that coating of immulectins to agarose beads enhanced cellular encapsulation in vitro. Immulectin-1 enhanced encapsulation but not melanization. Encapsulation occurred with isolated hemocytes that lack plasmatocytes when agarose beads were coated with immulectins. However, plasmatocytes were required for aggregation of encapsulated beads. We also showed that inhibitors of serine proteinases such as para-aminophenylmethylsulfonyl fluoride (p-APMSF) inhibited cellular encapsulation of immulectin-coated agarose beads. Our results suggest that humoral pattern recognition receptors may enhance cellular encapsulation and melanization. (c) 2005 Elsevier Ltd. All rights reserved.