Insight into cross-talk between intra-amoebal pathogens
Insight into cross-talk between intra-amoebal pathogens
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DOI：
10.1186/1471-2164-12-542
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发表时间：
2011-11-02
期刊：
影响因子：
4.4
通讯作者：
Greub, Gilbert
中科院分区：
文献类型：
--
作者：
Gimenez, Gregory;Bertelli, Claire;Greub, Gilbert
背景：变形虫是一种吞噬性原生生物，其耐阿米巴细菌之间可能发生遗传交换。这些变形虫病原体能够逃脱宿主的吞噬行为。它们属于不同的细菌门，并且通常显示出比人类感染病原体更大的基因组大小。这一特点被提出是频繁的基因交换与其他细菌，共享一个同域的生活方式和对比严格的人类pathogenes.Results之间观察到的基因组减少的结果：我们测序的一个新的阿米巴病原体，军团菌drancourtii的基因组，并比较其基因内容的衣原体相关的细菌，棘阿米巴副衣原体。系统发育重建确定了7个潜在的水平基因转移（HGT）之间的两个阿米巴抗药性细菌，包括一个完整的操纵子的四个基因编码的ABC型转运蛋白。这些比较明确了棘阿米巴和嗜肺军团菌之间基因交换的潜在案例，以及军团菌目和衣原体目其他成员之间的基因交换。此外，9例代表可能的HGT之间的代表从军团菌目或衣原体目和成员的立克次体order.Conclusions：本研究确定了众多的基因交换细胞内军团菌和衣原体目细菌，这可能优先发生在常见的夹杂物在他们的阿米巴宿主。因此，它有助于提高我们对与其特定生活方式相关的阿米巴内基因特性的认识。
Background: Amoebae are phagocytic protists where genetic exchanges might take place between amoeba-resistant bacteria. These amoebal pathogens are able to escape the phagocytic behaviour of their host. They belong to different bacterial phyla and often show a larger genome size than human-infecting pathogens. This characteristic is proposed to be the result of frequent gene exchanges with other bacteria that share a sympatric lifestyle and contrasts with the genome reduction observed among strict human pathogens.Results: We sequenced the genome of a new amoebal pathogen, Legionella drancourtii, and compared its gene content to that of a Chlamydia-related bacterium, Parachlamydia acanthamoebae. Phylogenetic reconstructions identified seven potential horizontal gene transfers (HGTs) between the two amoeba-resistant bacteria, including a complete operon of four genes that encodes an ABC-type transporter. These comparisons pinpointed potential cases of gene exchange between P. acanthamoebae and Legionella pneumophila, as well as gene exchanges between other members of the Legionellales and Chlamydiales orders. Moreover, nine cases represent possible HGTs between representatives from the Legionellales or Chlamydiales and members of the Rickettsiales order.Conclusions: This study identifies numerous gene exchanges between intracellular Legionellales and Chlamydiales bacteria, which could preferentially occur within common inclusions in their amoebal hosts. Therefore it contributes to improve our knowledge on the intra-amoebal gene properties associated to their specific lifestyle.